血浆中游离和总色氨酸的荧光分光光度自动分析（作者译）

[Spectrofluorimetric automatised assay of free and total tryptophan in plasma (author's transl)].

作者信息

Escande C, Bousquet B, Dreux C

出版信息

Pathol Biol (Paris). 1978 Oct;26(7):453-7.

Abstract

An automated fluorometric method, including norharmane transformation of tryptophan (TRP) by formaldehyde and perhydrol, is applied to free and total TRP determination in human plasma. Separation of free TRP determination in human plasma. Separation of free TRP is achieved by ultrafiltration at pH 7,25 under an air/CO2 flow. The procedure here described is easy, rapid and very sensitive, allowing to work on microsamples if free TRP separation could be realized with them. A correct precision is obtained (CV : 2,8% for plasma repetability) and the specificity is quite good in plasma according to the negligible effect of tryptamine. Frequent values, calculated from 19 healthy subjects are [mean +/- tSE (risk 5%)]:--free TRP : 11,8 +/- 1,5 mumol/l;--total TRP : 52,2 +/- 19,7 mumol/l.

摘要

一种自动荧光法，包括通过甲醛和双氧水将色氨酸（TRP）转化为去甲哈尔满，用于测定人血浆中的游离TRP和总TRP。分离人血浆中的游离TRP。通过在空气/二氧化碳气流下于pH 7.25进行超滤来实现游离TRP的分离。此处描述的方法简便、快速且非常灵敏，如果能够用微量样品实现游离TRP的分离，那么就可以对微量样品进行检测。获得了正确的精密度（血浆重复性的CV：2.8%），并且根据色胺的可忽略不计的影响，该方法在血浆中的特异性相当好。从19名健康受试者计算得出的常见值为[平均值±tSE（5%风险）]：——游离TRP：11.8±1.5μmol/L；——总TRP：52.2±19.7μmol/L。