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苦槛蓝花、果提取物对李斯特菌、金黄色葡萄球菌、大肠杆菌、鼠伤寒沙门氏菌生长的影响

Effects of Flower and Fruit Extracts of Melastoma malabathricum Linn. on Growth of Pathogenic Bacteria: Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, and Salmonella typhimurium.

机构信息

Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

出版信息

Evid Based Complement Alternat Med. 2013;2013:459089. doi: 10.1155/2013/459089. Epub 2013 Apr 10.

Abstract

Melastoma malabathricum Linn. is a shrub that comes with beautiful pink or purple flowers and has berries-like fruits rich in anthocyanins. This study was carried out with the aim to evaluate the inhibitory activities of different concentrations of the M. malabathricum Linn. flower and fruit crude extracts against Listeria monocytogenes IMR L55, Staphylococcus aureus IMR S244, Escherichia coli IMR E30, and Salmonella typhimurium IMR S100 using the disc diffusion method. The lowest concentrations of the extracts producing inhibition zones against the test microorganisms were used to determine their minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). In addition, the growth of Listeria monocytogenes IMR L55 and Staphylococcus aureus IMR S244 grown in medium supplemented with the respective extracts at different temperatures (4°C, 25°C, and 37°C) and pHs (4, 6, 7, and 8) was determined.

摘要

野牡丹是一种灌木,开有美丽的粉红色或紫色花朵,其果实类似浆果,富含花色苷。本研究旨在评估不同浓度的野牡丹花和果实粗提取物对单核细胞增生李斯特菌 IMR L55、金黄色葡萄球菌 IMR S244、大肠杆菌 IMR E30 和鼠伤寒沙门氏菌 IMR S100 的抑制活性,采用纸片扩散法。使用产生抑制环的提取物的最低浓度来确定其最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。此外,还测定了在不同温度(4°C、25°C 和 37°C)和 pH 值(4、6、7 和 8)下,补充有各自提取物的培养基中生长的单核细胞增生李斯特菌 IMR L55 和金黄色葡萄球菌 IMR S244 的生长情况。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ae/3638579/ae501b22eed7/ECAM2013-459089.001.jpg

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