Moreira Diana, Gullón Beatriz, Gullón Patricia, Gomes Ana, Tavaria Freni
CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.
Food Funct. 2016 Jul 13;7(7):3273-82. doi: 10.1039/c6fo00553e.
Bioactive food packaging is an innovative approach for the prevention of the growth of food-spoilage microorganisms. Four active extracts from agroindustrial subproducts (Eucalyptus wood, almond shells, corn cobs and grape pomace) with demonstrated antioxidant activity have been investigated for bestowing antimicrobial activity to bioactive packaging. To carry out this evaluation, the antioxidant extracts were tested against five food pathogenic bacteria, namely, Escherichia coli, Pseudomonas aeruginosa, Listeria monocytogenes, Staphylococcus aureus and Salmonella spp. The results obtained showed that all the tested extracts inhibited the growth of all five pathogenic bacteria. From the analysis of the minimal bactericidal concentrations (MBCs), the Eucalyptus wood extract was the most active, being necessary only 2% (v/v) to inhibit Pseudomonas aeruginosa, Listeria monocytogenes, and Staphylococcus aureus, whereas almond shells extract were less active requiring 4% (w/v) to inhibit the growth of Escherichia coli and Pseudomonas aeruginosa and the extract from corn cobs was bactericidal against Escherichia coli and Staphylococcus aureus at a concentration of 4% (w/v). After checking their antimicrobial activity, the antioxidant extracts have been incorporated into sodium alginate films and the maintenance of their antimicrobial properties was confirmed. This work showed that the antioxidant extracts from agroindustrial byproducts exhibited antimicrobial activity and were suitable for incorporation into edible films that could be used in bioactive packaging systems.
生物活性食品包装是一种预防食品腐败微生物生长的创新方法。已对四种具有抗氧化活性的农工业副产品（桉木、杏仁壳、玉米芯和葡萄皮渣）的活性提取物进行研究，以赋予生物活性包装抗菌活性。为进行该评估，针对五种食源致病菌，即大肠杆菌、铜绿假单胞菌、单核细胞增生李斯特菌、金黄色葡萄球菌和沙门氏菌属，对抗氧化提取物进行了测试。所得结果表明，所有测试提取物均抑制了所有五种致病菌的生长。通过对最低杀菌浓度（MBC）的分析，桉木提取物活性最高，仅需2%（v/v）即可抑制铜绿假单胞菌、单核细胞增生李斯特菌和金黄色葡萄球菌，而杏仁壳提取物活性较低，需要4%（w/v）才能抑制大肠杆菌和铜绿假单胞菌的生长，玉米芯提取物在浓度为4%（w/v）时对大肠杆菌和金黄色葡萄球菌具有杀菌作用。在检查其抗菌活性后，将抗氧化提取物掺入海藻酸钠薄膜中，并证实了其抗菌性能的维持。这项工作表明，农工业副产品的抗氧化提取物具有抗菌活性，适合掺入可用于生物活性包装系统的可食用薄膜中。
