Centre for Plant Molecular Biology, Osmania University, Hyderabad 500007, India.
J Biotechnol. 2013 Jul 10;166(3):114-21. doi: 10.1016/j.jbiotec.2013.04.016. Epub 2013 May 9.
Brassica juncea Nonexpressor of pathogenesis-related genes 1 (BjNPR1) has been introduced into commercial indica rice varieties by Agrobacterium-mediated genetic transformation. Transgenic rice plants were regenerated from the phosphinothricin-resistant calli obtained after co-cultivation with Agrobacterium strain LBA4404 harbouring Ti plasmid pSB111-bar-BjNPR1. Molecular analyses confirmed the stable integration and expression of BjNPR1 in various transgenic rice lines. Transgenes NPR1 and bar were stably inherited and disclosed co-segregation in subsequent generations in a Mendelian fashion. Homozygous transgenic rice lines expressing BjNPR1 protein displayed enhanced resistance to rice blast, sheath blight and bacterial leaf blight diseases. Rice transformants with higher levels of NPR1 revealed notable increases in plant height, panicle length, flag-leaf length, number of seeds/panicle and seed yield/plant as compared to the untransformed plants. The overall results amply demonstrate the profound impact of BjNPR1 in imparting resistance against major pathogens of rice. The multipotent BjNPR1, as such, seems promising as a prime candidate gene to fortify crop plants with durable resistance against various pathogens.
将 Brassica juncea Nonexpressor of pathogenesis-related genes 1（BjNPR1）基因通过农杆菌介导的遗传转化引入商业籼稻品种。通过与携带 Ti 质粒 pSB111-bar-BjNPR1 的农杆菌菌株 LBA4404 共培养获得抗草铵膦的愈伤组织，从中再生出转基因水稻植株。分子分析证实了 BjNPR1 在各种转基因水稻系中的稳定整合和表达。NPR1 和 bar 转基因稳定遗传，并在后代表现出孟德尔式的共分离。表达 BjNPR1 蛋白的纯合转基因水稻系表现出对稻瘟病、纹枯病和细菌性条斑病的增强抗性。与未转化的植物相比，NPR1 水平较高的水稻转化体表现出明显增加的株高、穗长、旗叶长、每穗粒数和单株产量。总的来说，这些结果充分证明了 BjNPR1 在赋予水稻对主要病原体抗性方面的深远影响。因此，多能的 BjNPR1 似乎是一个有前途的候选基因，可以为作物提供对各种病原体的持久抗性。
