College of Life Sciences, Hebei University, Baoding, Hebei 071002, PR China.
Gene. 2013 Aug 1;525(1):18-25. doi: 10.1016/j.gene.2013.04.067. Epub 2013 May 9.
To explore Lgr5 as the possible stem cell marker in human gastric tissue, 259 normal gastric tissues and dissected gastric adenocarcinoma were analyzed by immunohistochemistry, immunofluorescence double staining and qRT-PCR. The results demonstrated that Lgr5 was expressed in the bottom of the normal gastric gland units, and showed a differential expression in gastric adenocarcinoma with varying differentiation. Lgr5 and Bmi1 were co-expressed within the same cells of gastric glands. CD26+, CD44+, ALDH1+ and CD133+ cells co-existed with Lgr5+ cells in the stem cell zone of adjacent normal gastric mucosa, and they were detectable in gastric adenocarcinoma but behaved differently. We concluded that Lgr5 may be the adult stem cell marker in human gastric epithelium; Lgr5 and Bmi1 may belong to the same stem cell population; Lgr5, CD26, CD44, ALDH1, and CD133 may be functionally-associated.
为了探索 Lgr5 是否可以作为人胃组织中的干细胞标志物，我们通过免疫组化、免疫荧光双重染色和 qRT-PCR 分析了 259 例正常胃组织和解剖胃腺癌。结果表明，Lgr5 在正常胃腺单位的底部表达，并在不同分化程度的胃腺癌中呈现出差异表达。Lgr5 和 Bmi1 在胃腺内的同一细胞中共同表达。CD26+、CD44+、ALDH1+和 CD133+细胞与相邻正常胃黏膜干细胞区的 Lgr5+细胞共存，它们在胃腺癌中也可检测到，但表现不同。我们得出结论，Lgr5 可能是人胃上皮的成体干细胞标志物；Lgr5 和 Bmi1 可能属于同一干细胞群体；Lgr5、CD26、CD44、ALDH1 和 CD133 可能具有功能相关性。
