利用 mariner 转座子载体对脆弱拟杆菌进行转座子诱变。
Transposon mutagenesis of Bacteroides fragilis using a mariner transposon vector.
机构信息
GLAVAHCS, Los Angeles, CA 90073, USA.
出版信息
Anaerobe. 2013 Aug;22:126-9. doi: 10.1016/j.anaerobe.2013.04.012. Epub 2013 May 7.
Abstract
The mariner transposon vector pYV07 was tested for use in the mutagenesis of Bacteroides fragilis 638R. The transposon vector efficiently generated mutants in B. fragilis 638R. The transposon disrupted genes were scattered throughout the genome of B. fragilis 638R. This method serves as a powerful tool to study B. fragilis.
摘要
航海者转座子载体 pYV07 被测试用于脆弱拟杆菌 638R 的诱变。该转座子载体能有效地在脆弱拟杆菌 638R 中产生突变体。转座子破坏的基因散布在脆弱拟杆菌 638R 的基因组中。该方法是研究脆弱拟杆菌的有力工具。
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