Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic.
Int J Biol Macromol. 2013 Aug;59:396-401. doi: 10.1016/j.ijbiomac.2013.04.076. Epub 2013 May 7.
From the aerial parts of sage (Salvia officinalis L.) an arabino-(4-O-methyl-glucurono)-xylan (AGX) was isolated by alkaline extraction followed by precipitation with barium hydroxide solution. Polymer was isolated from sage as a light brown polysaccharide material of molecular mass (Mp) 84,000. Compositional analyses of sage AGX revealed xylose (81%), arabinose (10%), glucuronic acid (8%) and small amounts of hexoses (1%). Linkage sugar analyses showed the (1→4)-linked xylopyranosyl backbone with low degree of substitution (9-10%) at O-2 and O-3. Arabinofuranose residues were found as the terminal, 1,3-, 1,5- and 1,3,5-linked. NMR structural analyses of acidic oligomers, generated by partial acidic hydrolysis of AGX, confirmed a substitution of xylose residues by glucuronic acid and its 4-O-methyl derivate at O-2 at an average on every fourteenth xylose residue. NMR and FT-IR measurements, as well as a high negative optical rotation confirmed the β configuration of glycosidic linkages in AGX backbone.
从鼠尾草（Salvia officinalis L.）的地上部分，通过碱性提取，然后用氢氧化钡溶液沉淀，分离出阿拉伯糖-(4-O-甲基-葡萄糖醛酸)-木聚糖（AGX）。将 AGX 从鼠尾草中分离出来，得到一种浅棕色的多糖物质，分子量（Mp）为 84,000。鼠尾草 AGX 的组成分析表明，其含有 81%的木糖、10%的阿拉伯糖、8%的葡萄糖醛酸和少量的己糖（1%）。连接糖分析表明，（1→4）-连接的木吡喃糖主链在 O-2 和 O-3 位的取代度较低（9-10%）。阿拉伯呋喃糖残基作为末端、1,3-、1,5-和 1,3,5-连接。通过 AGX 的部分酸性水解生成的酸性低聚物的 NMR 结构分析，证实了木糖残基在 O-2 位被葡萄糖醛酸及其 4-O-甲基衍生物取代，平均每 14 个木糖残基取代一次。NMR 和 FT-IR 测量以及高负旋光性证实了 AGX 主链中糖苷键的β构型。
