Capek P, Hríbalová V, Svandová E, Ebringerová A, Sasinková V, Masarová J
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic.
Int J Biol Macromol. 2003 Nov;33(1-3):113-9. doi: 10.1016/s0141-8130(03)00075-8.
Crude polysaccharide fractions, rich mainly in arabinogalactans (A), pectin (B) and glucuronoxylan-related polymers (D), have been obtained from aerial parts of sage (Salvia officinalis L.) by sequential extraction with various reagents. Arabinogalactans displayed on HPLC a dominance of lower molecular-mass polymers (MW < 10,000), while pectin and glucuronoxylan-related polysaccharides showed predominance of polymers with MW > 50,000. Individual polysaccharide fractions were examined for their immunomodulatory activity in the in vitro comitogenic thymocyte test. The polysaccharide fractions tested possessed the capacity to induce rat thymocyte proliferation in the order D>B>A. Besides, fraction D possessed a significant comitogenic effect, and the SIcomit/SImit ratio 3-4 indicates potential adjuvant properties of this glucuronoxylan-rich material.
通过用各种试剂进行顺序提取，从鼠尾草（Salvia officinalis L.）的地上部分获得了主要富含阿拉伯半乳聚糖（A）、果胶（B）和葡糖醛酸木聚糖相关聚合物（D）的粗多糖级分。阿拉伯半乳聚糖在高效液相色谱上显示低分子量聚合物（MW < 10,000）占主导地位，而果胶和葡糖醛酸木聚糖相关多糖则显示MW > 50,000的聚合物占优势。在体外促有丝分裂胸腺细胞试验中检测了各个多糖级分的免疫调节活性。所测试的多糖级分具有诱导大鼠胸腺细胞增殖的能力，顺序为D>B>A。此外，级分D具有显著的促有丝分裂作用，SIcomit/SImit比值为3 - 4表明这种富含葡糖醛酸木聚糖的物质具有潜在的佐剂特性。
