Muthana Musleh M, Qu Jingyao, Xue Mengyang, Klyuchnik Timofey, Siu Alex, Li Yanhong, Zhang Lei, Yu Hai, Li Lei, Wang Peng G, Chen Xi
Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA.
Chem Commun (Camb). 2015 Mar 18;51(22):4595-8. doi: 10.1039/c4cc10306h.
Arabidopsis thaliana glucuronokinase (AtGlcAK) was cloned and shown to be able to use various uronic acids as substrates to produce the corresponding uronic acid-1-phosphates. AtGlcAK or Bifidobacterium infantis galactokinase (BiGalK) was used with a UDP-sugar pyrophosphorylase, an inorganic pyrophosphatase, with or without a glycosyltransferase for highly efficient synthesis of UDP-uronic acids and glucuronides. These improved cost-effective one-pot multienzyme (OPME) systems avoid the use of nicotinamide adenine dinucleotide (NAD(+))-cofactor in dehydrogenase-dependent UDP-glucuronic acid production processes and can be broadly applied for synthesizing various glucuronic acid-containing molecules.
拟南芥葡萄糖醛酸激酶（AtGlcAK）被克隆出来，并证明能够使用各种糖醛酸作为底物来生成相应的糖醛酸-1-磷酸。AtGlcAK或婴儿双歧杆菌半乳糖激酶（BiGalK）与尿苷二磷酸糖焦磷酸化酶、无机焦磷酸酶一起使用，有或没有糖基转移酶，用于高效合成尿苷二磷酸糖醛酸和葡糖醛酸苷。这些改进的具有成本效益的一锅多酶（OPME）系统避免了在依赖脱氢酶的尿苷二磷酸葡糖醛酸生产过程中使用烟酰胺腺嘌呤二核苷酸（NAD(+)）辅因子，并且可广泛应用于合成各种含葡糖醛酸的分子。
