Huang Yun, Wang Xueqian, Wang Xin, Xu Man, Liu Mei, Liu Dong
Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, 226001 Nantong, China.
Gene Expr Patterns. 2013 Oct;13(7):265-70. doi: 10.1016/j.gep.2013.04.005. Epub 2013 May 9.
Nonmuscle myosin II (NM II) is the name given to the multi-subunit protein product of three genes (myh9, myh10, and myh14) encoding different nonmuscle myosin heavy chains. The three NM II isoforms share a very similar molecular structure and play important roles in a variety of fundamental biological processes. NM II-B (myh10) has been shown to be essential for the formation of mouse neural system and heart. But so far the complete knowledge for its expression in developing zebrafish embryos is lacking. In current study, we proved the conservation of zebrafish NM II-B in vertebrate evolution by in silicon analysis. Afterwards the NM II-B (myh10) expression was demonstrated to initiate after gastrulation stage. At 20 hpf, the expression is mainly restricted in central nervous system (CNS). It was maintained and expanded to sensor organ including eye, otic vesicle, and olfactory bulb at 36 hpf and later. We also detected myh10 mRNA hybridization signal in 48 hpf zebrafish heart. In addition, we investigated myh9a and myh9b mRNA distribution in zebrafish developing embryos. It was shown that myh10 and myh9 have distinct expression pattern, with myh9s not in neural system but in epidermis, enveloping layer (EVL). Our study provides new insight into the NM II expression and the use of this model organism to tackle future studies on the role of NM II in embryo development.
非肌肉肌球蛋白II（NM II）是由三个基因（myh9、myh10和myh14）编码的不同非肌肉肌球蛋白重链的多亚基蛋白产物的名称。三种NM II同工型具有非常相似的分子结构，并在各种基本生物学过程中发挥重要作用。已证明NM II-B（myh10）对小鼠神经系统和心脏的形成至关重要。但到目前为止，尚缺乏其在斑马鱼胚胎发育过程中表达的完整知识。在当前的研究中，我们通过硅分析证明了斑马鱼NM II-B在脊椎动物进化中的保守性。随后证明NM II-B（myh10）的表达在原肠胚形成阶段后开始。在受精后20小时（hpf），表达主要局限于中枢神经系统（CNS）。在36 hpf及之后，它持续存在并扩展到感觉器官，包括眼睛、耳泡和嗅球。我们还在48 hpf斑马鱼心脏中检测到myh10 mRNA杂交信号。此外，我们研究了myh9a和myh9b mRNA在斑马鱼发育胚胎中的分布。结果表明，myh10和myh9具有不同的表达模式，myh9不在神经系统中表达，而是在表皮、包被层（EVL）中表达。我们的研究为NM II的表达以及利用这种模式生物解决未来关于NM II在胚胎发育中作用的研究提供了新的见解。
