Affiliated Hospital of Nantong University, Nantong Laboratory of Development and Diseases, School of Life Science; Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China.
Suqian First Hospital, Suqian, China.
Elife. 2024 Oct 4;13:RP95427. doi: 10.7554/eLife.95427.
Artificially sweetened beverages containing noncaloric monosaccharides were suggested as healthier alternatives to sugar-sweetened beverages. Nevertheless, the potential detrimental effects of these noncaloric monosaccharides on blood vessel function remain inadequately understood. We have established a zebrafish model that exhibits significant excessive angiogenesis induced by high glucose, resembling the hyperangiogenic characteristics observed in proliferative diabetic retinopathy (PDR). Utilizing this model, we observed that glucose and noncaloric monosaccharides could induce excessive formation of blood vessels, especially intersegmental vessels (ISVs). The excessively branched vessels were observed to be formed by ectopic activation of quiescent endothelial cells (ECs) into tip cells. Single-cell transcriptomic sequencing analysis of the ECs in the embryos exposed to high glucose revealed an augmented ratio of capillary ECs, proliferating ECs, and a series of upregulated proangiogenic genes. Further analysis and experiments validated that reduced mediated the excessive angiogenesis induced by monosaccharides via upregulating the expression of . This study has provided new evidence showing the negative effects of noncaloric monosaccharides on the vascular system and the underlying mechanisms.
人工甜味饮料中含有不含热量的单糖，被认为是含糖饮料的更健康替代品。然而，这些不含热量的单糖对血管功能的潜在不良影响仍未得到充分理解。我们建立了一种斑马鱼模型，该模型表现出由高葡萄糖诱导的显著过度血管生成，类似于增殖性糖尿病视网膜病变（PDR）中观察到的hyperangiogenic 特征。利用该模型，我们观察到葡萄糖和不含热量的单糖可以诱导血管过度形成，特别是节间血管（ISVs）。过度分支的血管是由静止的内皮细胞（ECs）异位激活为尖端细胞形成的。对暴露于高葡萄糖的胚胎中 ECs 的单细胞转录组测序分析显示，毛细血管 ECs、增殖 ECs 的比例增加，以及一系列上调的促血管生成基因。进一步的分析和实验验证了，通过上调 的表达，减少了单糖诱导的过度血管生成。这项研究提供了新的证据，表明不含热量的单糖对血管系统的负面影响及其潜在机制。
