Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, North 13, West 8, Kita-ku, Sapporo, Hokkaido 060-8628, Japan.
Bioresour Technol. 2013 Jul;139:285-91. doi: 10.1016/j.biortech.2013.04.018. Epub 2013 Apr 20.
In this study, partial nitrifying (PN) aerobic granules were developed in a sequencing batch airlift reactor by controlling the airflow rate and NH4(+) loading rate. The PN reactor produced an effluent with a NO2(-)/NH4(+) ratio of approximately one and with an NH4(+) conversion rate of 1.22 kg N m(-3)day(-1). More than 95% of the total organic carbon was removed during the process. On the basis of clone library analysis and fluorescence in situ hybridization, ammonia-oxidizing bacteria (AOB) closely related to Nitrosomonas eutropha and putative heterotrophic denitrifiers were mainly present near the surface of the PN aerobic granules. Microelectrode measurements revealed that both NH4(+) and NO2(-) were consumed near the surface (<200 μm), whereas no nitrate (NO3(-)) accumulation was observed throughout the granules. These results indicate that PN by AOB and nitrite denitrification by heterotrophs, but not nitrite oxidation, simultaneously occurred near the surface of the PN aerobic granules.
在这项研究中，通过控制气流速率和 NH4(+) 负载率，在序批式气升式反应器中开发了部分硝化 (PN) 好氧颗粒。PN 反应器产生的出水具有约为一的 NO2(-)/NH4(+) 比值，且 NH4(+) 的转化率为 1.22 kg N m(-3)day(-1)。在此过程中，超过 95%的总有机碳被去除。基于克隆文库分析和荧光原位杂交，与 Nitrosomonas eutropha 密切相关的氨氧化细菌 (AOB) 和可能的异养反硝化菌主要存在于 PN 好氧颗粒的表面附近。微电极测量表明，NH4(+) 和 NO2(-) 在表面附近（<200 μm）被消耗，而整个颗粒中均未观察到硝酸盐 (NO3(-)) 积累。这些结果表明，AOB 的 PN 和异养反硝化的亚硝酸盐还原，而不是亚硝酸盐氧化，同时发生在 PN 好氧颗粒的表面附近。
