Medicinal Plant Program, The Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA, USA.
Mol Biol Rep. 2013 Jul;40(7):4269-74. doi: 10.1007/s11033-013-2510-x. Epub 2013 May 12.
Plant identification, classification, and genotyping within a germplasm collection are essential elements for establishing a breeding program that enhances the probability of plants with desirable characteristics in the market place. In this study, random amplified polymorphic DNA (RAPD) was used as a molecular tool to assess the diversity and relationship among 20 summer squash (Curcubita pepo L.) landraces traditionally used to treat hypertension and prostate hyperplasia. A total of 10 RAPD primers produced 65 reproducible bands of which 46 (70.77 %) were polymorphic, indicating a large number of genotypes within the summer squash lines. Cluster analysis divided the summer squash germplasm into two groups, one including one landrace and a second containing 19 landraces that could be divided into five sub-groups. Results of this study indicate the potential of RAPD markers for the identification and assessment of genetic variations among squash landraces and provide a number of choices for developing a successful breeding program to improve summer squash.
在种质资源收集内进行植物鉴定、分类和基因分型是建立一个增强市场上具有理想特征的植物的可能性的育种计划的基本要素。在这项研究中，随机扩增多态性 DNA（RAPD）被用作分子工具，以评估 20 种传统用于治疗高血压和前列腺增生的南瓜（Cucurbita pepo L.）地方品种的多样性和关系。总共使用 10 个 RAPD 引物产生了 65 个可重复的条带，其中 46 个（70.77％）是多态性的，表明南瓜品系内存在大量基因型。聚类分析将南瓜种质分为两组，一组包括一个地方品种，另一组包括 19 个地方品种，可分为五个亚组。本研究的结果表明，RAPD 标记在鉴定和评估南瓜地方品种的遗传变异方面具有潜力，并为开发成功的育种计划以改善南瓜提供了多种选择。
