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利用形态特征和分子标记对地中海气候下生长的一些分类群进行表征

Characterization of Some Taxa Grown under Mediterranean Climate Using Morphological Traits and Molecular Markers.

作者信息

El-Taher Ahmed M, Elzilal Hala A, Abd El-Raouf Hany S, Mady Emad, Alshallash Khalid S, Alnefaie Rasha M, Mahdy Ehab M B, Ragab Osama G, Emam Elhassan A, Alaraidh Ibrahim A, Randhir Timothy O, Ibrahim Mohamed F M

机构信息

Department of Agricultural Botany, Faculty of Agriculture, Al-Azhar University, Cairo 11884, Egypt.

Department of Science and Technology, College of Ranyah, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.

出版信息

Plants (Basel). 2023 Jan 13;12(2):388. doi: 10.3390/plants12020388.

Abstract

The verification of taxonomic identities is of the highest significance in the field of biological study and categorization. Morpho-molecular characterization can clarify uncertainties in distinguishing between taxonomic groups. In this study, we characterized five local taxa of the genus using morphological and molecular markers for taxonomic authentication and probably future genetic improvement. The five taxa grown under the Mediterranean climate using morphological traits and molecular markers showed variations. The examined taxa showed a widespread range of variations in leaf characteristics, i.e., shape, type, texture, margin, and apex and cypsela characteristics i.e., shape, color, and surface pattern. The phylogenetic tree categorized the var. and var. in a single group, whereas var. was grouped separately. However, var. and subsp. were classified as a cluster. The recorded variance between classes using the molecular markers SCoT, ISSR, and RAPD was documented at 34.43%, 36.62%, and 40.34%, respectively. Authentication using molecular tools proved the usefulness of a dichotomous indented key, as revealed by morphological identification. The integrated methodology using morphological and molecular assessment could support improved verification and authentication of the various taxa of chicory. It seems likely that the Egyptian chicory belongs to subsp. .

摘要

在生物学研究和分类领域,分类身份的验证具有至关重要的意义。形态分子特征可以澄清分类群之间区分的不确定性。在本研究中,我们使用形态学和分子标记对该属的五个本地分类群进行特征描述,以进行分类鉴定并可能用于未来的遗传改良。在地中海气候条件下生长的这五个分类群,利用形态特征和分子标记显示出变异。所研究的分类群在叶片特征(即形状、类型、质地、边缘和顶端)以及瘦果特征(即形状、颜色和表面图案)方面表现出广泛的变异。系统发育树将变种和变种归为一个单一的组,而变种则单独分组。然而,变种和亚种被归为一个聚类。使用分子标记SCoT、ISSR和RAPD记录的类间方差分别为34.43%、36.62%和40.34%。如形态鉴定所示,使用分子工具进行鉴定证明了二歧缩进检索表的有用性。使用形态学和分子评估的综合方法可以支持对菊苣各种分类群进行更好的验证和鉴定。埃及菊苣似乎属于亚种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f6c/9866365/8052bd937f66/plants-12-00388-g001.jpg

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