Department of Environmental Conservation, University of Massachusetts, Amherst, MA, 01003, USA.
Horticulture Department, Faculty of Agriculture, Al-Azhar University, Nasr City, Cairo, Egypt.
Mol Biol Rep. 2022 May;49(5):3863-3873. doi: 10.1007/s11033-022-07233-3. Epub 2022 Feb 21.
Germplasm identification is an essential connection linking the conservation and exploitation of crop genetic resources in several plant breeding programs. This study highlights the biochemical and molecular variations in a collection of pumpkin genotypes representing four climate zones. The information could help improve germplasm management and sustainable exploitation of the neglected genotypes.
Chemical characterization and genetic diversity among nine Egyptian landraces of pumpkin (Cucurbita moschata Duchesne) were estimated using Diode Array (DDA) Near Infra-Red (NIR) technology and the Inter simple Sequence Repeat markers (ISSR). Pumpkin seeds were collected from various geographical parts of Egypt. The spectroscopic properties of pumpkin seeds were used to quantify the fat, moisture, protein, ash, fiber, and total carbohydrate contents. The ten ISSR primers generated a total number of 46 genotype-specific bands, and the total polymorphism produced in the tested landraces was 63.58%. Based on the ISSR data, the polymorphism analysis divided the nine pumpkin landraces into two main groups, two subgroups, and four sub subgroups. The most diverse pumpkin landraces were Alexandria and Sohag, with a similarity percentage of 49.6%. However, the highest calculated similarity value was 88.3% between Matruh and Gharbia. The resultant genotype-specific bands can be used as markers for future genotypic characterization of pumpkins.
The study results could be helpful in the chemical phenotypic characterization and the parental selection and planning for future breeding programs for pumpkin improvement.
种质鉴定是连接作物遗传资源保护和利用的重要环节，在多个植物育种计划中都具有重要意义。本研究重点关注了代表四个气候带的南瓜基因型群体中的生化和分子变异。这些信息可以帮助改进种质管理和对被忽视基因型的可持续利用。
采用二极管阵列（DDA）近红外（NIR）技术和简单重复间序列标记（ISSR），对埃及 9 个南瓜地方品种（Cucurbita moschata Duchesne）的化学特性和遗传多样性进行了评估。南瓜种子从埃及各地收集而来。南瓜种子的光谱特性用于定量测定脂肪、水分、蛋白质、灰分、纤维和总碳水化合物的含量。使用 10 个 ISSR 引物共产生了 46 个基因型特异性条带，在测试的地方品种中产生的总多态性为 63.58%。基于 ISSR 数据，多态性分析将 9 个南瓜地方品种分为两个主要组、两个亚组和四个亚组。最具多样性的南瓜地方品种是亚历山大和索哈格，相似度为 49.6%。然而，马图鲁和盖尔比亚之间的计算相似度最高为 88.3%。产生的基因型特异性条带可用于未来南瓜基因型特征的标记。
研究结果有助于进行化学表型特征分析，以及为未来的南瓜改良育种计划选择和规划亲本。
