米氏酮对培养的中国仓鼠细胞的细胞分裂、染色体数目和结构的影响。

The effect of Michler's ketone on cell division, chromosome number and structure in cultured Chinese hamster cells.

作者信息

Lafi A, Parry J M, Parry E M

机构信息

Department of Genetics, University College of Swansea, UK.

出版信息

Mutagenesis. 1986 Jan;1(1):17-20. doi: 10.1093/mutage/1.1.17.

DOI:10.1093/mutage/1.1.17

PMID:3325729

Abstract

The industrial chemical Michler's ketone (MK) has been examined for its ability to induce abnormalities of mitotic cell division and the production of chromosomal aberrations and aneuploidy. MK was shown to produce aberrant cell division stages in cultured mammalian cells probably by interference with centrosome replication leading to the production of monopolar spindles during metaphase, and multipolar telophase spindles, in the first division after exposure. Cells in the second division after exposure to MK showed increased levels of chromosome aneuploidy which is presumed to result from aberrant cell division. There was also some evidence of increased levels of chromosome structural aberrations at concentrations of 1.5 microgram/ml.

摘要

已对工业化学品米氏酮（MK）诱导有丝分裂细胞分裂异常以及产生染色体畸变和非整倍体的能力进行了研究。结果表明，MK可能通过干扰中心体复制，从而在中期导致单极纺锤体的产生，并在暴露后的第一次分裂中导致多极末期纺锤体的产生，进而在培养的哺乳动物细胞中产生异常细胞分裂阶段。暴露于MK后的第二次分裂中的细胞显示出染色体非整倍体水平升高，这被认为是异常细胞分裂的结果。在浓度为1.5微克/毫升时，也有一些证据表明染色体结构畸变水平有所增加。