Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603 203, Tamil Nadu, India.
Colloids Surf B Biointerfaces. 2013 Sep 1;109:294-300. doi: 10.1016/j.colsurfb.2013.04.006. Epub 2013 Apr 19.
Bone tissue engineering is a promising alternative method for treating bone loss by a combination of biomaterials and cells. In this study, we fabricated biocomposite scaffolds by blending chitosan (CS), alginate (Alg) and nano-silica (nSiO2), followed by freeze drying. The prepared scaffolds (CS/Alg, CS/Alg/nSiO2) were characterized by SEM, FT-IR and XRD analyses. In vitro studies such as swelling, biodegradation, biomineralization, protein adsorption and cytotoxicity were also carried out. The scaffolds possessed a well-defined porous architecture with pore sizes varying from 20 to 100 μm suitable for cell infiltration. The presence of nSiO2 in the scaffolds facilitated increased protein adsorption and controlled swelling ability. The scaffolds were biodegradable and the addition of nSiO2 improved apatite deposition on these scaffolds. There was no significant cytotoxicity effect of these CS/Alg/nSiO2 scaffolds towards osteolineage cells. Thus, these results indicate that CS/Alg/nSiO2 scaffolds may have potential applications for bone tissue engineering.
骨组织工程是一种有前途的方法，通过生物材料和细胞的结合来治疗骨丢失。在这项研究中，我们通过混合壳聚糖（CS）、海藻酸盐（Alg）和纳米二氧化硅（nSiO2），然后进行冷冻干燥来制备生物复合材料支架。通过 SEM、FT-IR 和 XRD 分析对制备的支架（CS/Alg、CS/Alg/nSiO2）进行了表征。还进行了体外研究，如溶胀、生物降解、生物矿化、蛋白质吸附和细胞毒性。支架具有定义明确的多孔结构，孔径从 20 到 100μm，适合细胞渗透。支架中 nSiO2 的存在促进了蛋白质的吸附和对溶胀能力的控制。支架是可生物降解的，并且添加 nSiO2 可改善这些支架上的磷灰石沉积。这些 CS/Alg/nSiO2 支架对成骨细胞没有明显的细胞毒性作用。因此，这些结果表明 CS/Alg/nSiO2 支架可能在骨组织工程中有潜在的应用。
