Kim Hye-Lee, Jung Gil-Yong, Yoon Jun-Ho, Han Jung-Suk, Park Yoon-Jeong, Kim Do-Gyoon, Zhang Miqin, Kim Dae-Joon
Department of Advanced Materials Engineering, Sejong University, 209 Neungdong-ro, Gwangjin-gu, Seoul 143-747, South Korea.
Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 152-742, South Korea.
Mater Sci Eng C Mater Biol Appl. 2015 Sep;54:20-5. doi: 10.1016/j.msec.2015.04.033. Epub 2015 Apr 22.
The aim of this study was to develop chitosan composite scaffolds with high strength and controlled pore structures by homogenously dispersed nano-sized hydroxyapatite (nano-HAp) powders. In the fabrication of composite scaffolds, nano-HAp powders distributed in an alginate (AG) solution with a pH higher than 10 were mixed with a chitosan (CS) solution and then freeze dried. While the HAp content increased up to 70 wt.%, the compressive strength and the elastic modulus of the composite scaffolds significantly increased from 0.27 MPa and 4.42 MPa to 0.68 MPa and 13.35 MPa, respectively. Higher content of the HAp also helped develop more differentiation and mineralization of the MC3T3-E1 cells on the composite scaffolds. The uniform pore structure and the excellent mechanical properties of the HAp/CS composite scaffolds likely resulted from the use of the AG solution at pH 10 as a dispersant for the nano-HAp powders.
本研究的目的是通过均匀分散纳米级羟基磷灰石（nano-HAp）粉末来制备具有高强度和可控孔结构的壳聚糖复合支架。在复合支架的制备过程中，将分散在pH高于10的藻酸盐（AG）溶液中的纳米HAp粉末与壳聚糖（CS）溶液混合，然后进行冷冻干燥。当HAp含量增加到70 wt.%时，复合支架的抗压强度和弹性模量分别从0.27 MPa和4.42 MPa显著增加到0.68 MPa和13.35 MPa。较高含量的HAp也有助于在复合支架上使MC3T3-E1细胞产生更多的分化和矿化。HAp/CS复合支架均匀的孔结构和优异的力学性能可能源于使用pH为10的AG溶液作为纳米HAp粉末的分散剂。
