Faculty of Chemistry, Jagiellonian University, Kraków, Poland.
Colloids Surf B Biointerfaces. 2013 Sep 1;109:307-16. doi: 10.1016/j.colsurfb.2013.03.059. Epub 2013 Apr 22.
Stable vesicles for efficient curcumin encapsulation, delivery and controlled release have been obtained by coating of liposomes with thin layer of newly synthesized chitosan derivatives. Three different derivatives of chitosan were obtained and studied: the cationic (by introduction of the stable, quaternary ammonium groups), the hydrophobic (by attachment of N-dodecyl groups) and cationic-hydrophobic one (containing both quaternary ammonium and N-dodecyl groups). Zeta potential measurements confirmed effective coating of liposomes with all these chitosan derivatives. The liposomes coated with cationic-hydrophobic chitosan derivative are the most promising curcumin carriers; they can easily penetrate cell membrane and release curcumin in a controlled manner. Biological studies indicated that such systems are non-toxic for murine fibroblasts (NIH3T3) while toxic toward murine melanoma (B16F10) cell line.
通过在脂质体上涂覆薄层新合成的壳聚糖衍生物，获得了用于有效包封、递送和控制释放姜黄素的稳定囊泡。研究了三种不同的壳聚糖衍生物：阳离子型（通过引入稳定的季铵基团）、疏水性（通过连接 N-十二烷基基团）和阳离子-疏水性（同时含有季铵和 N-十二烷基基团）。Zeta 电位测量证实了所有这些壳聚糖衍生物对脂质体的有效包覆。用阳离子-疏水性壳聚糖衍生物包覆的脂质体是最有前途的姜黄素载体；它们可以很容易地穿透细胞膜并以可控的方式释放姜黄素。生物学研究表明，这些系统对鼠成纤维细胞（NIH3T3）无毒，而对鼠黑素瘤（B16F10）细胞系有毒。
