Samoĭlina N L, Gan O I, Medvinskiĭ A L
Ontogenez. 1990 Mar-Apr;21(2):177-84.
Localization and time of appearance as well as dynamics of quantitative changes of splenic colony-forming units (CFU-S) in mouse (C57BL/6 X CBA)F1 embryos were studied. Cells taken from the whole embryo (day 8), yolk sac and embryo per se (day 9), and also liver (day 10) were injected into the lethally irradiated syngenic mice. 7-8 days after the injection the spleens were fixed and the number of macrocolonies was counted. Statistically significant number of CFU-S was detected starting from day 10 of development, first in the embryo (30-33 somites), then in yolk sac and blood (37-38 somites) and liver (after the 40 somites stage). Rapid increase of CFU-S number during days 11-12 (two-fold increase in about 4.6 hours) suggest that not only active proliferation of CFU-S but also maturation of CFU-S precursors take place.
研究了小鼠（C57BL/6×CBA）F1胚胎中脾集落形成单位（CFU-S）的定位、出现时间以及数量变化动态。从整个胚胎（第8天）、卵黄囊和胚胎本身（第9天）以及肝脏（第10天）获取细胞，注射到经致死性照射的同基因小鼠体内。注射后7 - 8天，固定脾脏并计数大集落的数量。从发育第10天开始检测到具有统计学意义的CFU-S数量，首先在胚胎（30 - 33体节）中，然后在卵黄囊和血液（37 - 38体节）以及肝脏（40体节阶段之后）中。在第11 - 12天期间CFU-S数量迅速增加（约4.6小时内增加两倍），这表明不仅CFU-S有活跃增殖，而且CFU-S前体细胞也在成熟。
