Pan Yung-Wei, Wang Wenbin, Xia Zhengui
Graduate Program, Molecular and Cellular Biology, University of Washington, Seattle, Washington, USA.
Curr Protoc Toxicol. 2013 May;Chapter 12:Unit12.20. doi: 10.1002/0471140856.tx1220s56.
Adult neurogenesis is a lifelong developmental process that occurs in two discrete regions in the adult mammalian brain: the subgranular zone of the dentate gyrus (DG) and the subventricular zone (SVZ) along the lateral ventricles. Despite immense interest in the therapeutic potential of adult neural stem cells (aNSCs) residing along these two neurogenic regions, molecular and cellular mechanisms regulating this process are not fully defined. Defining the regulatory mechanisms responsible for the genesis of new neurons in the adult brain is integral to understanding the basic biology of aNSCs. The techniques described here provide a basic blueprint to isolate, culture, and perform experiments using aNSCs in vitro as well as providing methods to perform immunohistochemistry on brain sections. Curr. Protoc. Toxicol. 56:12.20.1-12.20.16. © 2013 by John Wiley & Sons, Inc.
成体神经发生是一个终身的发育过程，发生在成年哺乳动物大脑的两个离散区域：齿状回（DG）的颗粒下区和侧脑室周围的室下区（SVZ）。尽管人们对存在于这两个神经发生区域的成体神经干细胞（aNSCs）的治疗潜力有着浓厚兴趣，但调节这一过程的分子和细胞机制尚未完全明确。明确负责成年大脑中新神经元生成的调节机制对于理解aNSCs的基本生物学特性至关重要。本文所述技术提供了一个基本蓝图，用于体外分离、培养aNSCs并进行实验，同时还提供了对脑切片进行免疫组织化学的方法。《毒理学实验指南》56:12.20.1 - 12.20.16。© 2013约翰威立父子公司。
