Center for Preventive Doping Research/Institute of Biochemistry, German Sport University Cologne, Germany.
Proteomics Clin Appl. 2013 Aug;7(7-8):561-70. doi: 10.1002/prca.201300009. Epub 2013 Jul 9.
Gamma irradiation of red blood cell (RBC) concentrates is routinely used to prevent transfusion-associated graft-versus-host disease. So far, the effects of ionizing radiation on RBC structure and function and especially the proteome are not fully understood.
RBC concentrates were irradiated with 30 Gy and stored for 1 or 15 days at 4 ± 2°C. Following cell lysis and hemoglobin depletion, 2D-DIGE was used to examine the changes of the cytosolic RBC proteome. Significantly altered spots were analyzed using bottom-up proteomic approaches and selected marker proteins validated by western blotting.
Gamma irradiation was found to enhance conventional RBC storage lesions. Following 15 days of postirradiation storage, the abundances of a total of 27 spots were significantly altered and 3 out of 13 identified proteins were selected and validated as potential marker proteins for the assessment of irradiation-induced cytosolic RBC lesions.
Gamma irradiation and subsequent ex vivo storage according to the Council of Europe guidelines were found to affect RBC protein structures. The validated marker proteins can serve as a basis for the development of a screening assay to monitor the quality of irradiated RBC concentrates during ex vivo storage.
辐照红细胞（RBC）浓缩物是常规用于预防输血相关移植物抗宿主病的方法。到目前为止，电离辐射对 RBC 结构和功能的影响，尤其是对蛋白质组的影响还不完全清楚。
用 30Gy 辐照 RBC 浓缩物，并在 4°C±2°C 下储存 1 或 15 天。在细胞裂解和血红蛋白耗尽后，使用 2D-DIGE 检测细胞质 RBC 蛋白质组的变化。使用基于 Bottom-up 的蛋白质组学方法分析明显改变的斑点，并通过 Western blot 验证选择的标记蛋白。
辐照被发现增强了常规的 RBC 储存损伤。辐照后储存 15 天，总共 27 个斑点的丰度明显改变，其中 3 个被鉴定的蛋白质被选为潜在的标记蛋白，用于评估辐照诱导的细胞质 RBC 损伤。
辐照和随后根据欧洲委员会指南进行的体外储存被发现会影响 RBC 蛋白质结构。验证的标记蛋白可作为开发筛选检测的基础，以监测体外储存期间辐照 RBC 浓缩物的质量。
