修饰干细胞的基因不稳定性——迈向恶性转化的第一步？
Genetic instability of modified stem cells - a first step towards malignant transformation?
作者信息
Steinemann Doris, Göhring Gudrun, Schlegelberger Brigitte
机构信息
Institute of Cell and Molecular Pathology, Hannover Medical School Hannover, Germany.
出版信息
Am J Stem Cells. 2013 Mar 8;2(1):39-51. Print 2013.
Abstract
Induced pluripotent stem cells (iPSC) are important tools in regenerative medicine. Yet, it is becoming increasingly clear that the reprogramming process, including retroviral transduction with potent oncogenes like c-Myc and long-term cultivation, may induce genetic instability. Genetically altered iPS cells can grow out and dominate the cell culture. This review intends to comprehensively summarize the current knowledge on genetic instability of embryonic and iPSCs, with an emphasis on cytogenetic alterations, and compares these data with what is known from tumorigenesis.
摘要
诱导多能干细胞（iPSC）是再生医学中的重要工具。然而，越来越明显的是，重编程过程，包括用c-Myc等强效致癌基因进行逆转录病毒转导以及长期培养，可能会诱导基因不稳定。基因改变的iPS细胞能够生长并主导细胞培养。本综述旨在全面总结目前关于胚胎干细胞和诱导多能干细胞基因不稳定的知识，重点关注细胞遗传学改变，并将这些数据与肿瘤发生的已知情况进行比较。
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