National Research Council, Ottawa, ON, Canada K1A 0R6.
Carbohydr Res. 2013 Aug 30;378:108-13. doi: 10.1016/j.carres.2013.04.010. Epub 2013 Apr 18.
Elucidation of the carbohydrate backbone structure of the lipopolysaccharide (LPS) from Piscirickettsia salmonis, the etiological agent of the salmonid rickettsial septicemia, is described. Structural information was established by a combination of monosaccharide and methylation analyses of LPS, and by NMR and mass spectrometries of oligosaccharides obtained through the use of various chemical degradations of the native polymer. The following structure of the backbone sugars was determined on the basis of the combined data from these experiments: [formula see text] The presence of two consecutive residues of diacetylated pseudaminic acid (Pse5,7Ac, 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid) in the LPS appears to be unique among polysaccharides containing this acidic sugar. Similarly, the presence of 4-aminoarabinose (Ara4N, 4-amino-4-deoxy-l-arabinopyranose) on O-4 of the α-GlcN1P of the lipid A moiety is a unique feature of this LPS.
已阐明鲑鱼鱼立克次氏体（引起鲑鱼传染性败血病的病原体）脂多糖（LPS）的碳水化合物主链结构。通过对 LPS 的单糖和甲基化分析以及通过使用各种天然聚合物的化学降解获得的寡糖的 NMR 和质谱分析，结合这些实验的综合数据确定了以下主链糖的结构：[公式见正文]在含有这种酸性糖的多糖中，LPS 中连续存在两个二乙酰化 pseudaminic 酸（Pse5,7Ac，5,7-二乙酰基-3,5,7,9-四脱氧-l-甘油-l-甘露诺-2-酮酸）残基，这似乎是独特的。同样，脂质 A 部分的α-GlcN1P 的 O-4 上存在 4-氨基阿拉伯糖（Ara4N，4-氨基-4-去氧-l-阿拉伯吡喃糖）也是该 LPS 的独特特征。
