Xu G X, Xie X H, Liu F Y, Zang D L, Zheng D S, Huang D J, Huang M X
Shanghai Institute of Pharmaceutical Industry, State Pharmaceutical Administration of China.
Pharm Res. 1990 May;7(5):553-7. doi: 10.1023/a:1015837321087.
Four methods for encapsulating adenosine triphosphate (ATP) in liposomes were evaluated. Optimum entrapment required emulsifying ATP with the lipids used to form the liposome membrane in a high-speed homogenizer followed by evaporating the organic solvent with vigorous stirring. Under these optimum conditions ATP entrapment was 38.9%; i.e., the dosage form contained 38.9 g of ATP per 100 g of lipid. The distribution of positively charged liposomes loaded with ATP was studied in dogs with experimentally induced myocardial infarction. Intravenous injection of positively charged ATP liposomes caused accumulation of ATP in myocardial infarct tissue. Myocardial infarct tissue has reduced blood flow; since positively charged liposomes accumulated in infarct tissue, liposomes may be a drug delivery system for this disease state.
评估了四种将三磷酸腺苷（ATP）包裹于脂质体中的方法。最佳包封需要在高速匀浆器中将ATP与用于形成脂质体膜的脂质乳化，然后剧烈搅拌蒸发有机溶剂。在这些最佳条件下，ATP包封率为38.9%；即剂型中每100克脂质含有38.9克ATP。对实验性诱导心肌梗死犬体内负载ATP的带正电荷脂质体的分布进行了研究。静脉注射带正电荷的ATP脂质体导致ATP在心肌梗死组织中蓄积。心肌梗死组织血流减少；由于带正电荷的脂质体在梗死组织中蓄积，脂质体可能是针对这种疾病状态的一种药物递送系统。
