Institute for Research in Biomedicine, Baldiri i Reixac 10, 08028 Barcelona, Spain.
Org Biomol Chem. 2013 Jun 28;11(24):4109-21. doi: 10.1039/c3ob40492g.
Given the growing importance of drug and gene delivery systems, imaging agents, biosensors, and theranostics, there is a need to develop new multifunctional and biocompatible platforms. Here we synthesized and fully characterized a family of novel multifunctional and completely monodisperse dendritic platforms. Our synthetic methodology, based on compatible protecting groups and the attachment of monodisperse triethylene glycol units, allows the control of the generation and differentiation of terminal groups, thus giving rise to multifunctional and perfectly-defined products. A family of dendrons was synthesized and four distinct dendritic structures were chosen from the family in order to determine the effect of the generation and surface groups on their biocompatibility. The stability in serum, cytotoxicity, and hemocompatibility of these products were studied. Our results indicate that these non-toxic, hemocompatible, non-immunogenic, stable and versatile scaffolds may be very interesting candidates for biomedical applications.
鉴于药物和基因传递系统、成像剂、生物传感器和治疗学的重要性日益增加，需要开发新的多功能和生物相容的平台。在这里，我们合成并充分表征了一系列新型多功能且完全单分散的树枝状平台。我们的合成方法基于兼容的保护基团和单分散三乙二醇单元的连接，允许控制末端基团的生成和分化，从而产生多功能和完全定义的产物。合成了一系列树突，并从该系列中选择了四种不同的树枝状结构，以确定生成和表面基团对其生物相容性的影响。研究了这些产物在血清中的稳定性、细胞毒性和血液相容性。我们的结果表明，这些无毒、血液相容、非免疫原性、稳定且多功能的支架可能是非常有前途的生物医学应用候选物。
