Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
J Lipid Res. 2013 Jul;54(7):2023-8. doi: 10.1194/jlr.D038794. Epub 2013 May 14.
In this study, a method of supercritical fluid extraction (SFE) with carbon dioxide of polyisoprenoids from plant photosynthetic tissues is described. SFE was an effective extraction method for short- and medium-chain compounds with even higher yield than that observed for the "classical extraction" method with organic solvents. Moreover, SFE-derived extracts contained lower amounts of impurities (e.g., chlorophylls) than those obtained by extraction of the same tissue with organic solvents. Elevated temperature and extended extraction time of SFE resulted in a higher rate of extraction of long-chain polyisoprenoids. Ethanol cofeeding did not increase the extraction efficiency of polyisoprenoids; instead, it increased the content of impurities in the lipid extract. Optimization of SFE time and temperature gives the opportunity of prefractionation of complex polyisoprenoid mixtures accumulated in plant tissues. Extracts obtained with application of SFE are very stable and free from organic solvents and can further be used directly in experimental diet supplementation or as starting material for preparation of semisynthetic polyisoprenoid derivatives, e.g., polyisoprenoid phosphates.
本研究描述了一种从植物光合组织中提取类异戊二烯的超临界流体萃取（SFE）方法。SFE 是一种有效提取短链和中链化合物的方法，其产率甚至高于有机溶剂的“经典提取”方法。此外，SFE 衍生的提取物比用有机溶剂提取相同组织获得的提取物含有更少的杂质（例如叶绿素）。升高 SFE 的温度和延长提取时间会导致长链类异戊二烯的提取率更高。乙醇共进料不会增加类异戊二烯的提取效率；相反，它会增加脂质提取物中杂质的含量。SFE 时间和温度的优化为在植物组织中积累的复杂类异戊二烯混合物的预分级提供了机会。应用 SFE 获得的提取物非常稳定，不含有机溶剂，可直接用于实验性饮食补充，或用作制备半合成类异戊二烯衍生物（例如类异戊二烯磷酸盐）的起始材料。
