Ibrahim F A, Ali F A, Ahmed S M, Tolba M M
Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt.
Int J Biomed Sci. 2007 Mar;3(1):20-30.
A simple and sensitive kinetic spectrophotometric method was established for the determination of acarbose and miglitol in bulk and in their pharmaceutical preparations using alkaline potassium permanganate as an oxidizing agent. The method involves determination of acarbose and miglitol by kinetic studies of their oxidation at room temperature for a fixed time of 15 minutes for acarbose and 25 minutes for miglitol. The absorbance of the colored manganate ion was measured at 610 nm. Alternatively, the kinetic decrease in the absorbance of permanganate upon addition of the studied drugs at 525 nm was also used. The absorbance concentration plot was rectilinear over the concentration range of 4-20 and 1-10 μg/ml for acarbose and miglitol, respectively. The detection limits were 0.189 and 0.089 μg/ml at 610 nm and 0.081 and 0.179 μg/ml at 525 nm for acarbose and miglitol respectively. The method was successfully applied for the determination of these drugs in their dosage forms. The results obtained were in good agreement with those obtained with the reference methods.
建立了一种简单灵敏的动力学分光光度法，以碱性高锰酸钾为氧化剂，用于测定原料药及其制剂中的阿卡波糖和米格列醇。该方法通过在室温下对阿卡波糖氧化15分钟、米格列醇氧化25分钟的动力学研究来测定阿卡波糖和米格列醇。在610nm处测量有色锰酸根离子的吸光度。另外，也可在525nm处测量加入被测药物后高锰酸钾吸光度的动力学降低情况。阿卡波糖和米格列醇的吸光度-浓度曲线在4-20μg/ml和1-10μg/ml浓度范围内分别呈直线。阿卡波糖在610nm处的检测限为0.189μg/ml，在525nm处为0.081μg/ml；米格列醇在610nm处的检测限为0.089μg/ml，在525nm处为0.179μg/ml。该方法成功应用于这些药物剂型的测定。所得结果与参考方法的结果吻合良好。
