1981年古巴登革热出血热/登革休克综合征流行期间分离出的登革2型病毒株的生物学和分子特性
Biological and Molecular Properties of Dengue 2 Strains Isolated during the DHF/DSS Cuban Epidemic, 1981.
作者信息
González Lester, Alvarez Mayling, Del Rosario Delfina, Pavón Alequis, Prado Irina, Díaz Joel, Morier Luis, Guzmán María G
机构信息
Department of Virology, PAHO/WHO Collaborating Centre for the Study of Dengue and its Vector, Institute of Tropical Medicine "Pedro Kourí", Havana City, Cuba.
出版信息
Int J Biomed Sci. 2007 Jun;3(2):137-43.
To study some biological and molecular properties of nine DENV-2 strains isolated during the 1981 Cuban epidemic, temperature sensitivity, viral plaque size, the kinetic of virus replication in newborn mice inoculated by intracerebral route, the influence of pH medium on virus-cell attachment phase and the restriction enzyme pattern were studied. Strains were classified in two patterns according to temperature sensitivity, plaque size, and virus replication in mouse brain and cell culture and restriction enzymatic pattern the changes observed differentiate clearly the strains isolated at the beginning and at the end of the epidemic suggesting that viruses with different characteristics circulated.
为研究1981年古巴疫情期间分离出的9株登革热病毒2型（DENV-2）的一些生物学和分子特性，对温度敏感性、病毒蚀斑大小、经脑内途径接种新生小鼠后病毒复制的动力学、pH培养基对病毒-细胞附着阶段的影响以及限制性酶切图谱进行了研究。根据温度敏感性、蚀斑大小、病毒在小鼠脑和细胞培养中的复制情况以及限制性酶切图谱，将这些毒株分为两种模式。观察到的变化清楚地区分了疫情开始和结束时分离出的毒株，这表明有不同特性的病毒在传播。
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