Morens D M, Halstead S B, Repik P M, Putvatana R, Raybourne N
J Clin Microbiol. 1985 Aug;22(2):250-4. doi: 10.1128/jcm.22.2.250-254.1985.
A newly modified semimicro plaque reduction neutralization test (PRNT) in BHK cells was compared with a standard PRNT in bottles with LLC-MK2 monolayers and with an LLC-MK2 PRNT adapted to semimicro methods. The BHK semimicro PRNT compared favorably in terms of sensitivity in detecting dengue antibody (96%), specificity at a screening dilution (95%), and ability to detect seroconversion to dengue viruses of three serotypes (93%). Disagreements between the BHK test and the LLC-MK2 tests were attributed to greater sensitivity of the BHK test in detecting dengue type 2 (DEN-2) antibody in acute-phase sera and to apparent low-level DEN-1/DEN-3 cross-reactions in some sera in all three tests. The BHK PRNT was easier, faster, and more economical than either of the LLC-MK2 tests. Many of the benefits of the BHK PRNT derive from the fact that cells are infected while still in suspension, at the time of cell splitting, hence the term "BHK suspension test."
将在BHK细胞中一种新改良的半微量蚀斑减少中和试验（PRNT）与在装有LLC-MK2单层细胞的培养瓶中的标准PRNT以及适应半微量方法的LLC-MK2 PRNT进行比较。BHK半微量PRNT在检测登革热抗体的敏感性（96%）、筛查稀释度下的特异性（95%）以及检测三种血清型登革热病毒血清转化的能力（93%）方面表现良好。BHK试验与LLC-MK2试验之间的差异归因于BHK试验在检测急性期血清中2型登革热（DEN-2）抗体时具有更高的敏感性，以及在所有三项试验中某些血清中明显存在的低水平DEN-1/DEN-3交叉反应。BHK PRNT比LLC-MK2试验中的任何一种都更简便、快速且经济。BHK PRNT的许多优点源于这样一个事实，即在细胞传代时，细胞仍处于悬浮状态时就被感染，因此称为“BHK悬浮试验”。
