Guzman M G, Deubel V, Pelegrino J L, Rosario D, Marrero M, Sariol C, Kouri G
Virology Department, Pedro Kouri Tropical Medicine Institute, Havana, Cuba.
Am J Trop Med Hyg. 1995 Mar;52(3):241-6. doi: 10.4269/ajtmh.1995.52.241.
In 1981, an epidemic of dengue hemorrhagic fever (DHF) caused by dengue-2 virus occurred in Cuba. This was the first DHF epidemic reported in the Western Hemisphere. In this study, we have analyzed four dengue-2 Cuban strains for two short genomic fragments: one on the envelope (E) glycoprotein and one at the E/nonstructural protein-1 (NS1) gene junction. The E segment of these 1981 Cuban isolates were more closely related to older dengue-2 virus strains such as New Guinea C 1944, Thailand 1964, Sri Lanka 1968, and Burma 1976 than to more recent isolates of this virus from Jamaica and Vietnam. More than 9% of the divergence with strains isolated from Jamaica and Vietnam was observed at the E/NS1 gene junction. One nucleotide change was observed between the first strain isolated during the epidemic and the rest of the Cuban strains. This mutation induced a nonconserved amino acid change from phenylalanine to leucine at position 43 that was not observed in any of the other strains with which it was compared.
1981年，古巴发生了由登革2型病毒引起的登革出血热（DHF）疫情。这是西半球报告的首例DHF疫情。在本研究中，我们分析了4株古巴登革2型病毒的两个短基因组片段：一个位于包膜（E）糖蛋白上，另一个位于E/非结构蛋白1（NS1）基因连接处。这些1981年古巴分离株的E片段与较老的登革2型病毒株（如1944年的新几内亚C株、1964年的泰国株、1968年的斯里兰卡株和1976年的缅甸株）的关系比与该病毒最近从牙买加和越南分离的毒株更为密切。在E/NS1基因连接处，观察到与从牙买加和越南分离的毒株有超过9%的差异。在疫情期间分离的第一株毒株与其他古巴毒株之间观察到一个核苷酸变化。这种突变在第43位诱导了一个从苯丙氨酸到亮氨酸的非保守氨基酸变化，在与之比较的任何其他毒株中均未观察到。
