Department of Chemistry, Faculty of Science, Assiut University, Assiut, 71516, Egypt.
Upper Egypt Pharmaceuticals (UP Pharma), Industrial Zone, Arab Al-Awamer, Assiut, Egypt.
Anal Sci. 2024 Nov;40(11):1997-2004. doi: 10.1007/s44211-024-00632-5. Epub 2024 Aug 25.
A high-performance liquid chromatographic method (HPLC) with UV detection is described for determination of ceftriaxone sodium (CFX) and cefotaxime sodium (CFM) content in pharmaceutical industrial wastewater. These methods are based on the detection of these antibiotics via the formation of chelate complexes with Cu(II). The developed Liquid Chromatographic method offers symmetric peak shape, good resolution and reasonable retention time for both drugs. The removal percentage reached about 100 and 92.1% at pH 7.2 for CFX and CFM, respectively. In UV detection, the removal of the chelating antibiotics were based on forming of chelate complexes with Cu(II) which detected at λ 253 and 244 nm for CFX and CFM, respectively. Linearity, accuracy and precision were found to be acceptable over the concentration range of 5.99-59.86 µg mL for CFX and 14.33-71.63 µg mL for CFM. The proposed method can be used for the quality control of industrial wastewater containing CFX and CFM.
描述了一种高效液相色谱法（HPLC），结合紫外检测，用于测定制药工业废水中头孢曲松钠（CFX）和头孢噻肟钠（CFM）的含量。这些方法基于通过与 Cu(II) 形成螯合配合物来检测这些抗生素。所开发的液相色谱法为两种药物提供了对称的峰形、良好的分辨率和合理的保留时间。在 pH 7.2 下，CFX 和 CFM 的去除率分别达到约 100%和 92.1%。在紫外检测中，螯合抗生素的去除是基于与 Cu(II) 形成螯合配合物，分别在 λ 253 和 244nm 处检测到 CFX 和 CFM。发现 CFX 的浓度范围为 5.99-59.86µg mL 时，CFM 的浓度范围为 14.33-71.63µg mL 时，线性、准确性和精密度均令人满意。该方法可用于含有 CFX 和 CFM 的工业废水的质量控制。
