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BD SurePath 和 Hologic PreservCyt 液基细胞学介质中人基因组和人乳头瘤病毒 DNA 的长期稳定性。

Long-term stability of human genomic and human papillomavirus DNA stored in BD SurePath and Hologic PreservCyt liquid-based cytology media.

机构信息

BD Diagnostics, Sparks, Maryland, USA.

出版信息

J Clin Microbiol. 2013 Aug;51(8):2702-6. doi: 10.1128/JCM.00759-13. Epub 2013 May 15.

DOI:10.1128/JCM.00759-13
PMID:23678069
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3719621/
Abstract

We evaluated the effect of storage at 2 to 8°C on the stability of human genomic and human papillomavirus (HPV) DNA stored in BD SurePath and Hologic PreservCyt liquid-based cytology media. DNA retained the ability to be extracted and PCR amplified for more than 2.5 years in both medium types. Prior inability to detect DNA in archived specimens may have been due to failure of the extraction method to isolate DNA from fixed cells.

摘要

我们评估了在 2 至 8°C 下储存对保存在 BD SurePath 和 Hologic PreservCyt 液体细胞学介质中的人类基因组和人乳头瘤病毒 (HPV) DNA 稳定性的影响。在这两种介质类型中，DNA 都能保持 2.5 年以上的可提取和 PCR 扩增能力。以前无法在存档标本中检测到 DNA 可能是由于提取方法未能从固定细胞中分离出 DNA。

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