Bangalore University, Bangalore, India.
Biol Trace Elem Res. 2011 Sep;142(3):623-37. doi: 10.1007/s12011-010-8780-4. Epub 2010 Jul 24.
Multigenerational evaluation was made in rats on exposure to high fluoride (100 and 200 ppm) to assess neurotoxic potential of fluoride in discrete areas of the brain in terms of lipid peroxidation and the activity of antioxidant enzyme system. The rats were given fluoride through drinking water (100 and 200 ppm) and maintained subsequently for three generations. Fluoride treatment significantly increased the lipid peroxidation and decreased the activity of antioxidant enzymes viz, catalase, superoxide dismutase, glutathione peroxidase, glutathione S-transferase, and glutathione level in first-generation rats and these alterations were more pronounced in the subsequent second and third-generation rats in both the doses tested. Decreased feed and water consumption, litter size and organ (brain) somatic index, marginal drop in body growth rate and mortality were observed in all three generations. Decreased antioxidant enzyme activity and increased malondialdehyde levels found in the present study might be related to oxidative damage that occurs variably in discrete regions of the brain. Results of this study can be taken as an index of neurotoxicity in rats exposed to water fluoridation over several generations.
对大鼠进行了多代暴露于高氟（100 和 200 ppm）的评估，以评估氟在大脑不同区域的神经毒性潜力，包括脂质过氧化和抗氧化酶系统的活性。大鼠通过饮用水（100 和 200 ppm）摄入氟，并随后维持三个世代。氟化物处理显著增加了第一世代大鼠的脂质过氧化作用，并降低了抗氧化酶的活性，如过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽 S-转移酶和谷胱甘肽水平，并且在两个测试剂量的随后的第二和第三代大鼠中这些变化更为明显。在所有三个世代中，都观察到饲料和水的摄入量减少、产仔数和器官（大脑）体指数减少、体重增长率略有下降和死亡率增加。本研究中发现的抗氧化酶活性降低和丙二醛水平升高可能与氧化损伤有关，这种损伤在大脑的不同区域以不同的方式发生。本研究的结果可以作为大鼠在几代水中氟化物暴露后神经毒性的指标。
