Okwuosa O M T B, Chukwura E I, Chukwuma G O, Okwuosa C N, Enweani I B, Agbakoba N R, Chukwuma C M, Manafa P O, Umedum C U
Department of Medical Laboratory Sciences, Nnamdi Azikiwe University, Awka Nnewi Campus, Nigeria.
Afr J Med Med Sci. 2012 Dec;41 Suppl:99-103.
The effects of crude and dilutions of aqueous, methanolic and n-hexane extracts of Uvaria chamae (roots and leaves), Spondias mombin (leaves and bark) and Combretum racemosum (Leaves), on pathogenic candida albicans and aspergillus niger was studied. The aim was to contribute to the search for a cheaper, conventional cure for both fungi. Phytochemical analysis revealed varying degrees of alkaloids, glycosides, saponin, lipid and oil, tannins, flavonoids, terpenoids and acids. Agar diffusion method was used for anti fungal assay. Minimum inhibitory concentration (mic) used was 10 mg/ml of extract and dilutions of the non polar solvents of 10 (-1) and 10(-2) was used. Results showed that none of the plant parts was active against aspergillus niger. Combretum racemosum had no antifungal effect on tested organisms as well as the different dilutions. However crude methanolic extract of uvaria (roots and leaves), spondias ( bark and leaves ), and n-hexane extracts of uvaria (leaves and roots), produced anti candidal effects with diameters in this order 14.67 +/- 0.72mm, 10.67 +/- 0.52 mm, 11.00 +/- 0.47 mm, 15.00 +/- 0.47 mm, and 14.67 +/- 0.72 mm respectively. Some of the plant parts especially uvaria had zones of inhibition at a confidence limit comparable with control drug which is ketoconazole and it had inhibitory effects at a diameter of 20.06 +/- 0.40 mm.
研究了紫玉盘（根和叶）、酸豆（叶和树皮）和总序桤木（叶）的水提取物、甲醇提取物和正己烷提取物的粗提物及其稀释液对致病性白色念珠菌和黑曲霉的影响。目的是为寻找一种更便宜的、针对这两种真菌的传统治疗方法做出贡献。植物化学分析显示了不同程度的生物碱、糖苷、皂苷、脂质和油、单宁、黄酮类化合物、萜类化合物和酸。采用琼脂扩散法进行抗真菌试验。使用的最低抑菌浓度（mic）为提取物10mg/ml，并使用了10（-1）和10（-2）的非极性溶剂稀释液。结果表明，没有任何植物部位对黑曲霉有活性。总序桤木对受试生物体及其不同稀释液均无抗真菌作用。然而，紫玉盘（根和叶）的粗甲醇提取物、酸豆（树皮和叶）以及紫玉盘（叶和根）的正己烷提取物产生了抗念珠菌作用，直径依次为14.67±0.72mm、10.67±0.52mm、11.00±0.47mm、15.00±0.47mm和14.67±0.72mm。一些植物部位，特别是紫玉盘，在与对照药物酮康唑相当的置信限下有抑菌圈，其在直径为20.06±0.40mm时有抑制作用。
