Combretum album Pers. 叶提取物对某些病原菌的抗菌效果。
Antibacterial efficacy of leaf extracts of Combretum album Pers. against some pathogenic bacteria.
机构信息
Mosquito, Microbiology and Nanotechnology Research Units, Parasitology Laboratory, Department of Zoology, The University of Burdwan, Golapbag, Burdwan, West Bengal, 713104, India.
Department of Zoology, Durgapur Government College, JN Avenue, Durgapur, 713214, India.
出版信息
BMC Complement Altern Med. 2018 Jul 11;18(1):213. doi: 10.1186/s12906-018-2271-0.
BACKGROUND
Plant derived medicines show significant contributions to mankind in treating infections of pathogenic bacteria. Recently plants are used in pharmaceutical industries for novel drug preparations because to ensure efficacy and safety as synthetic antibiotics are threatened for their multidrug resistance. The present study aimed at finding antibacterial potential of aqueous and ethanolic leaf extracts of Combretum album.
METHODS
Antibacterial activity was evaluated against seven bacterial strains by determining minimum inhibitory concentration (MIC) and zone of inhibition. Diameters of the zone of inhibition were compared with standard antibiotics. Preliminary phytochemical screening was done according to standard protocol. FTIR analysis was performed to identify the general phytochemical groups of compounds in the extract. All experiments were conducted in triplicate and values were expressed as the mean ± standard deviation. One-way analysis of variance (ANOVA) and Tukey tests were performed for statistical justification.
RESULTS
Maximum zones of inhibition were found in case of ethanolic extracts in the following order Bacillus licheniformis (MTCC 530) > Pseudomonas aeruginosa (MTCC 2453) > Bacillus subtilis (MTCC 441) >, Pseudomonas fluorescens (MTCC 103) > Bacillus mycoides (MTCC 7343) > Escherichia coli (MTCC 739) > Pseudomonas putida (MTCC 1654) with zone of inhibition of 27.67 ± 0.33 mm diameter in B. licheniformis (MTCC 530). Qualitatively, the ethanol extract contains flavonoids, tannins and alkaloids. The results of FTIR analysis confirmed the presence of R-CH2-OH groups, aromatics, C-N stretching amine and NH stretching secondary amine. One way ANOVA and Tukey tests statistically justify the data (p ≤ 0.05).
CONCLUSIONS
All the tested leaf extracts showed promising antibacterial activity against both gram positive and gram negative bacteria. Phytochemical screening and FTIR analysis revealed the presence of tannins, alkaloids, R-CH2-OH groups, aromatics and flavonoids in ethanolic leaf extract qualitatively and these compounds could be responsible for antibacterial property of leaf extracts of C. album.
背景
植物来源的药物在治疗病原菌感染方面为人类做出了重大贡献。由于合成抗生素受到多药耐药性的威胁，其疗效和安全性难以保证，最近植物被应用于制药行业以制备新型药物。本研究旨在寻找白花酸藤果叶的水提物和醇提物的抗菌潜力。
方法
通过测定最小抑菌浓度（MIC）和抑菌圈直径来评估抗菌活性，以评估七种细菌菌株。抑菌圈直径与标准抗生素进行比较。根据标准方案进行初步的植物化学筛选。进行傅里叶变换红外光谱（FTIR）分析以鉴定提取物中化合物的一般植物化学基团。所有实验均重复三次，结果表示为平均值±标准差。采用单因素方差分析（ANOVA）和 Tukey 检验进行统计学验证。
结果
醇提物的抑菌圈直径最大，按以下顺序排列：地衣芽孢杆菌（MTCC 530）>铜绿假单胞菌（MTCC 2453）>枯草芽孢杆菌（MTCC 441）>荧光假单胞菌（MTCC 103）>蕈状芽孢杆菌（MTCC 7343）>大肠杆菌（MTCC 739）>恶臭假单胞菌（MTCC 1654），在地衣芽孢杆菌（MTCC 530）中的抑菌圈直径为 27.67±0.33mm。定性分析表明，乙醇提取物含有黄酮类、单宁类和生物碱类化合物。FTIR 分析结果证实存在 R-CH2-OH 基团、芳香族化合物、C-N 伸展胺和 NH 伸展仲胺。单因素方差分析和 Tukey 检验对数据进行了统计学验证（p≤0.05）。
结论
所有测试的叶提取物对革兰氏阳性菌和革兰氏阴性菌均显示出有希望的抗菌活性。植物化学筛选和 FTIR 分析表明，醇提物中存在单宁类、生物碱类、R-CH2-OH 基团、芳香族化合物和黄酮类化合物，这些化合物可能是白花酸藤果叶提取物具有抗菌活性的原因。
Zotero 插件