Kaboré Kayaba, Dibala Crépin Ibingou, Sama Hemayoro, Diao Mamounata, Somda Marius K, Dicko Mamoudou H
Laboratory of Biochemistry, Biotechnology, Food Technology and Nutrition, Department of Biochemistry and Microbiology, University Joseph Ki-ZERBO, Ouagadougou 09 BP 848, Burkina Faso.
Laboratory of Microbiology and Microbial Biotechnology, Department of Biochemistry and Microbiology, University Joseph Ki-ZERBO, Ouagadougou, Burkina Faso.
Biochem Res Int. 2024 Mar 26;2024:1289859. doi: 10.1155/2024/1289859. eCollection 2024.
The study aimed to evaluate phenolic content and antioxidant and antibacterial potentials of the fractions of the hydroethanolic extract of leaves, a food plant from Burkina Faso. Thus, the hexane, dichloromethane, ethyl acetate, and butanol fractions of the hydroalcoholic extract after drying were used to determine phenolic compound content, antioxidant activity, and antimicrobial potential on strains of pathogenic bacteria responsible for food contamination. Phytochemical analyses were performed according to standardized methods, while antioxidant activity was evaluated by DPPH and FRAP methods. The antibacterial activity of the fractions was determined by diffusion and microdilution methods on the agar medium with gentamicin as a reference antibiotic. All the six strains, namely, ATCC 19430, ATCC 8739, ATCC 25923, ATCC 9027, ATCC 13061, and ATCC 7644, were sensitive to the fractions tested. Minimum inhibitory concentrations ranged from 37 g·mL to 1.67 mg·mL, respectively, gentamicin and butanolic fractions, while minimum bactericidal concentrations of the fractions ranged from 0.037 to 2.500 mg·mL depending on the bacterial strain. Antioxidant activity varied significantly between fractions. For DPPH free radical scavenging activity, the butanol fraction was the most active, with an IC50 of 280 g/mL, while the lowest activity (705 g/mL) was recorded by the hexane fraction. Those of trolox and ascorbic acid used as standards were 80 and 100 g/mL, respectively. Ferric reducing power (FRAP) ranged from 0.34 to 0.40 mmol EAA/g extract for the hexanic and ethyl acetate fractions, respectively. Phenolic compound contents also varied significantly between fractions. Butanoic and ethyl acetate presented the best contents of total phenolics and flavonoids, respectively. Significant and positive correlations were also recorded between phenolics and antioxidant activities. The antibacterial and antioxidant activities of the active fractions would be related to their richness in bioactive compounds, including phenolic, which are powerful natural antioxidants. leaf extracts could therefore be used as dietary supplements to boost the immune system and prevent bacterial infections.
该研究旨在评估一种来自布基纳法索的食用植物叶片的水乙醇提取物各馏分中的酚类含量、抗氧化和抗菌潜力。因此，干燥后的水醇提取物的己烷、二氯甲烷、乙酸乙酯和丁醇馏分被用于测定酚类化合物含量、抗氧化活性以及对导致食品污染的病原菌菌株的抗菌潜力。植物化学分析按照标准化方法进行，而抗氧化活性通过DPPH和FRAP方法进行评估。各馏分的抗菌活性通过在琼脂培养基上的扩散法和微量稀释法测定，以庆大霉素作为参考抗生素。所有六种菌株，即ATCC 19430、ATCC 8739、ATCC 25923、ATCC 9027、ATCC 13061和ATCC 7644，对所测试的馏分均敏感。最低抑菌浓度分别为庆大霉素馏分37 μg·mL至丁醇馏分1.67 mg·mL，而各馏分的最低杀菌浓度根据细菌菌株不同在0.037至2.500 mg·mL之间。各馏分之间的抗氧化活性差异显著。对于DPPH自由基清除活性，丁醇馏分活性最高，IC50为280 μg/mL，而己烷馏分活性最低（705 μg/mL）。用作标准的Trolox和抗坏血酸的IC50分别为80和100 μg/mL。己烷馏分和乙酸乙酯馏分的铁还原能力（FRAP）分别为0.34至0.40 mmol EAA/g提取物。酚类化合物含量在各馏分之间也有显著差异。丁酸馏分和乙酸乙酯馏分分别呈现出最佳的总酚和黄酮含量。酚类与抗氧化活性之间也存在显著的正相关。活性馏分的抗菌和抗氧化活性可能与其生物活性化合物（包括酚类，酚类是强大的天然抗氧化剂）的丰富程度有关。因此，该植物叶片提取物可作为膳食补充剂来增强免疫系统并预防细菌感染。
