Sarikaya Buket Bozkurt, Berkov Strahil, Bastida Jaume, Kaya Gulen Irem, Onur Mustafa Ali, Somer Nehir Unver
Department of Pharmacognosy, Faculty of Pharmacy, Ege University, Bornova 35100, Izmir, Turkey.
Nat Prod Commun. 2013 Mar;8(3):327-8.
A GC-MS analysis of alkaloids in the aerial parts and bulbs of Galanthus xvalentinei nothosubsp. subplicatus was performed for the first time. Totally, twenty-six alkaloids were identified, of which tazettine and galanthindole were the major ones. Acetylcholinesterase inhibitory activity of the alkaloidal extracts was determined using modified in vitro Ellman's method. Significant anticholinesterase activity was observed in the tested samples (bulbs: IC50 = 21.3 microg/mL, aerial parts: IC50 = 16.3 microg/mL).
首次对皱叶雪滴花（Galanthus xvalentinei nothosubsp. subplicatus）地上部分和鳞茎中的生物碱进行了气相色谱-质谱联用（GC-MS）分析。共鉴定出26种生物碱，其中，七叶树碱和加兰吲哚为主要生物碱。采用改良的体外埃尔曼方法测定了生物碱提取物的乙酰胆碱酯酶抑制活性。在测试样品中观察到显著的抗胆碱酯酶活性（鳞茎：IC50 = 21.3微克/毫升，地上部分：IC50 = 16.3微克/毫升）。
