Rebijith K B, Asokan R, Kumar N K Krishna, Krishna V, Chaitanya B N, Ramamurthy V V
Division of Biotechnology, Indian Institute of Horticultural Research, Bangalore, India.
Bull Entomol Res. 2013 Oct;103(5):601-10. doi: 10.1017/S0007485313000278. Epub 2013 May 17.
Rapid, precise and timely identification of invasive pest insects such as aphids is important and a challenge worldwide due to their complex life cycles, parthenogenetic reproduction, sex and colour morphs. In this respect, DNA barcoding employing a 658 bp fragment of 5′ region of the mitochondrial cytochrome oxidase I (CO-I) gene is an effective tool in addressing the above. In the present study, we employed CO-I for discriminating 142 individuals representing 32 species of aphids from India. Sequence analyses revealed that the intraspecific and interspecific distances ranged from zero to 3.8% and 2.31 to 18.9%, respectively. In addition, the study also showed for the first time the prevalence of three cryptic species, namely Brevicoryne brassicae (Linnaeus), Hyperomyzus carduellinus (Theobald) and Brachycaudus helichrysi (Kaltenbach) from India. Our work has clearly demonstrated that DNA barcoding is an efficient and accurate method for identification of aphid species (including cryptic species), an approach that potentially could play an important role in formulating viable pest management strategies, more especially biocontrol.
由于蚜虫复杂的生命周期、孤雌生殖、性别和颜色形态，快速、准确且及时地识别诸如蚜虫这类入侵害虫在全球范围内都很重要且颇具挑战。在这方面，利用线粒体细胞色素氧化酶I（CO-I）基因5′区域的658 bp片段进行DNA条形码分析是解决上述问题的有效工具。在本研究中，我们利用CO-I对代表印度32种蚜虫的142个个体进行鉴别。序列分析表明，种内和种间距离分别为0至3.8%和2.31至18.9%。此外，该研究还首次显示了来自印度的三种隐存种的存在，即甘蓝蚜（林奈）、卡氏高暗斑蚜（西奥博尔德）和菊小长管蚜（卡尔滕巴赫）。我们的工作清楚地表明，DNA条形码分析是识别蚜虫物种（包括隐存种）的一种高效且准确的方法，这种方法可能在制定可行的害虫管理策略，尤其是生物防治策略方面发挥重要作用。
