Zhu Xi-Chao, Chen Jing, Chen Rui, Jiang Li-Yun, Qiao Ge-Xia
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, No. 1-5 Beichen West Road, Chaoyang District, Beijing 100101, P.R. China; College of Life Science, University of Chinese Academy of Sciences, Shijingshan District, Beijing 100049, P.R. China.
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, No. 1-5 Beichen West Road, Chaoyang District, Beijing 100101, P.R. China.
Zookeys. 2017 Feb 14(656):25-50. doi: 10.3897/zookeys.656.11440. eCollection 2017.
Chaitophorinae aphids are widespread across Eurasia and North America, and include some important agricultural and horticultural pests. So, accurate rapid species identification is very important. Here, we used three mitochondrial genes and one endosymbiont gene to calculate and analyze the genetic distances within different datasets. For species delimitation, two distance-based methods were employed, threshold with NJ (neighbor-joining) and ABGD (Automatic Barcode Gap Discovery), and two tree-based approaches, GMYC (General Mixed Yule Coalescent) and PTP (Poisson Tree Process). The genetic interspecific divergence was clearly larger than the intraspecific divergence for four molecular markers. COI and COII genes were found to be more suitable for Chaitophorinae DNA barcoding. For species delimitation, at least one distance-based method combined with one tree-based method would be preferable. Based on the data for and , DNA barcoding may also reveal geographical variation.
毛蚜亚科蚜虫广泛分布于欧亚大陆和北美洲，其中包括一些重要的农业和园艺害虫。因此，准确快速地进行物种鉴定非常重要。在此，我们使用三个线粒体基因和一个内共生体基因来计算和分析不同数据集中的遗传距离。对于物种界定，我们采用了两种基于距离的方法，即NJ（邻接法）阈值法和ABGD（自动条形码间隙发现法），以及两种基于树的方法，即GMYC（广义混合尤尔合并法）和PTP（泊松树过程法）。对于四个分子标记，种间遗传差异明显大于种内差异。发现COI和COII基因更适合毛蚜亚科的DNA条形码分析。对于物种界定，至少一种基于距离的方法与一种基于树的方法相结合会更好。基于[具体数据1]和[具体数据2]的数据，DNA条形码分析也可能揭示地理变异。
