National Environmental Health Program, Invertebrate Biodiversity, Agriculture and Agri-Food Canada, K. W. Neatby Bldg., 960 Carling Ave., Ottawa, ON, Canada K1A 0C6, Department of Biology, Utah State University, 5305 Old Main Hill, Logan, UT 84322, USA, Biodiversity Institute of Ontario, Department of Integrative Biology, University of Guelph, Guelph, ON, Canada N1G 2W1.
Mol Ecol Resour. 2008 Nov;8(6):1189-201. doi: 10.1111/j.1755-0998.2008.02297.x.
A 658-bp fragment of mitochondrial DNA from the 5' region of the mitochondrial cytochrome c oxidase 1 (COI) gene has been adopted as the standard DNA barcode region for animal life. In this study, we test its effectiveness in the discrimination of over 300 species of aphids from more than 130 genera. Most (96%) species were well differentiated, and sequence variation within species was low, averaging just 0.2%. Despite the complex life cycles and parthenogenetic reproduction of aphids, DNA barcodes are an effective tool for identification.
一段 658 个碱基对的线粒体 DNA 片段，来自线粒体细胞色素 c 氧化酶 1（COI）基因的 5'区域，已被采纳为动物生命的标准 DNA 条码区域。在这项研究中，我们测试了它在区分来自 130 多个属的 300 多种蚜虫方面的有效性。大多数（96%）物种得到了很好的区分，物种内的序列变异很小，平均只有 0.2%。尽管蚜虫的生命周期复杂，且具有孤雌生殖，但 DNA 条码是一种有效的鉴定工具。
