细胞培养中树突形态的半自动分析
Semiautomated analysis of dendrite morphology in cell culture.
作者信息
Sweet Eric S, Langhammer Chris L, Kutzing Melinda K, Firestein Bonnie L
机构信息
Neuroscience Graduate Program, Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ, USA.
出版信息
Methods Mol Biol. 2013;1018:261-8. doi: 10.1007/978-1-62703-444-9_24.
Abstract
Quantifying dendrite morphology is a method for determining the effect of biochemical pathways and extracellular agents on neuronal development and differentiation. Quantification can be performed using Sholl analysis, dendrite counting, and length quantification. These procedures can be performed on dendrite-forming cell lines or primary neurons grown in culture. In this protocol, we describe the use of a set of computer programs to assist in quantifying many aspects of dendrite morphology, including changes in total and localized arbor complexity.
摘要
量化树突形态是一种确定生化途径和细胞外因子对神经元发育和分化影响的方法。量化可以使用Sholl分析、树突计数和长度量化来进行。这些程序可以在培养的形成树突的细胞系或原代神经元上进行。在本方案中，我们描述了使用一组计算机程序来协助量化树突形态的许多方面，包括总体和局部树突复杂性的变化。
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