Monro J A, Bailey R W
Carbohydr Res. 1975 May;41:153-61. doi: 10.1016/s0008-6215(00)87015-9.
The extraction by alkali of hemicellulose polysaccharides and polymer hydroxyproline from non-delignified, primary cell-walls of lupin hypocotyls has been studied, using sequential extractions at 0 degrees and 18-22 degrees. 10% Aqueous potassium hydroxide at 0 degrees rapidly removed about two-thirds of the hemicellulose normally extracted in 10% KOH at 18-22 degrees and including nearly all of the hemicellulose-A. Little hydroxyproline was released at 0 degrees. When the temperature was subsequently raised to 18-22 degrees, the remaining 10% KOH-soluble hemicellulose, along with most of the hydroxyproline, was released. The monosaccharide composition of these fractions changed markedly with the time of extraction. Arabinose increased from 35% of the polysaccharide extracted during the first hour at 18-22 degrees to similar to 65% of the polysaccharide extracted between 16 and 20 h at 18-22 degrees. Hydroxyproline changed similarly as a proportion of polymer. The implications of these and other results are discussed in relation to polysaccharide and polymer extraction without prior delignification and to models of the primary cell-wall.
利用在0℃和18 - 22℃下的连续萃取，研究了从羽扇豆下胚轴未脱木质素的初生细胞壁中碱提取半纤维素多糖和聚合物羟脯氨酸的情况。0℃下10%的氢氧化钾水溶液迅速去除了通常在18 - 22℃下用10%氢氧化钾提取的约三分之二的半纤维素，包括几乎所有的半纤维素A。0℃时释放的羟脯氨酸很少。随后当温度升至18 - 22℃时，剩余的10%可溶于氢氧化钾的半纤维素以及大部分羟脯氨酸被释放出来。这些组分的单糖组成随提取时间显著变化。阿拉伯糖从在18 - 22℃下第一小时提取的多糖的35%增加到在18 - 22℃下16至20小时之间提取的多糖的约65%。羟脯氨酸作为聚合物的比例也有类似变化。结合多糖和聚合物在未预先脱木质素情况下的提取以及初生细胞壁模型，讨论了这些结果和其他结果的意义。
