O'Neill M A, Selvendran R R
Biochem J. 1985 Apr 15;227(2):475-81. doi: 10.1042/bj2270475.
The 1 M-KOH extract from the depectinated cell walls of parenchymatous tissues of mature runner bean (Phaseolus coccineus) on neutralization, dialysis and concentration gave insoluble (hemicellulose A) and soluble (hemicellulose B) carbohydrate complexes in the weight ratio 2:1. Both fractions contained polysaccharide, protein and polyphenolic material. The structural features of the carbohydrates were examined by methylation analysis. Hemicellulose A contained mainly pectic arabinogalactan, with lesser amounts of arabinoxylan and glucan. Sequential fractionation of hemicellulose B by anion-exchange and hydroxyapatite chromatography gave a range of polysaccharide-protein-polyphenolic complexes. The main polysaccharides in these complexes were (acidic) arabinoxylans, galactans, arabinogalactans 1 and 2 and xyloglucans. The proteins contained small amounts of hydroxyproline, but were rich in aspartic acid and glutamic acid. Attempts to determine the nature of the polyphenolic material were unsuccessful. The structural features of the polysaccharide-protein-polyphenolic complexes are discussed in relation to the structure of the cell walls of parenchymatous tissues.
从成熟的红花菜豆（Phaseolus coccineus）薄壁组织的去果胶细胞壁中提取的1M - KOH提取物，经中和、透析和浓缩后，得到了重量比为2:1的不溶性（半纤维素A）和可溶性（半纤维素B）碳水化合物复合物。这两个部分都含有多糖、蛋白质和多酚物质。通过甲基化分析研究了碳水化合物的结构特征。半纤维素A主要含有果胶阿拉伯半乳聚糖，还有少量阿拉伯木聚糖和葡聚糖。通过阴离子交换和羟基磷灰石色谱对半纤维素B进行连续分级分离，得到了一系列多糖 - 蛋白质 - 多酚复合物。这些复合物中的主要多糖是（酸性）阿拉伯木聚糖、半乳聚糖、阿拉伯半乳聚糖1和2以及木葡聚糖。蛋白质含有少量羟脯氨酸，但富含天冬氨酸和谷氨酸。确定多酚物质性质的尝试未成功。结合薄壁组织细胞壁的结构讨论了多糖 - 蛋白质 - 多酚复合物的结构特征。
