Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan 70101, Republic of China.
J Biomater Sci Polym Ed. 2013;24(9):1152-63. doi: 10.1080/09205063.2012.743061. Epub 2012 Nov 22.
Magnetic nanoparticles were the thermoseeds under an alternating magnetic field and can be used to produce highly localized hyperthermia effect on deep-seated tumor. Nevertheless, effective and precisive delivery of nanoparticles to the treatment-intended site remains a challenge. In this study, Fe3O4 nanoparticles were incorporated onto the crosslinked electrospun chitosan nanofibers using chemical co-precipitation from the Fe ions adsorbed. Such magnetic nanoparticle-nanofiber composites could be delivered to the treatment site precisely by surgical or endoscopic method. Iminodiacetic acid (IDA) functionality was grafted onto the chitosan with an aim to increase the amount of magnetic nanoparticles formed in the electrospun magnetic nanofiber composite. The morphology, crystalline phase as well as the magnetism characteristic of the magnetic electrospun nanofiber matrixes, was analyzed. Results have indicated that, with the incorporation of IDA functionality, more magnetic nanoparticles were formed in the electrospun chitosan nanofiber matrix. In addition, the magnetic IDA-grafted chitosan nanofiber composite can effectively reduced the tumor cell proliferation under the application of magnetic field. This finding suggested the magnetic electrospun chitosan nanofiber composite can be of potential for hyperthermia treatment.
磁性纳米粒子是交变磁场下的热种子，可用于产生深部肿瘤的高度局限的热疗效应。然而，将纳米粒子有效且精确地递送到治疗部位仍然是一个挑战。在这项研究中，通过吸附的 Fe 离子的化学共沉淀将 Fe3O4 纳米粒子掺入交联的静电纺丝壳聚糖纳米纤维中。这种磁性纳米粒子-纳米纤维复合材料可以通过手术或内窥镜方法精确地递送到治疗部位。亚氨基二乙酸（IDA）官能团被接枝到壳聚糖上，目的是增加静电纺丝磁性纳米纤维复合材料中形成的磁性纳米粒子的数量。分析了磁性静电纺纳米纤维基质的形态、晶相以及磁性特征。结果表明，随着 IDA 官能团的掺入，更多的磁性纳米粒子在静电纺丝壳聚糖纳米纤维基质中形成。此外，磁性IDA 接枝壳聚糖纳米纤维复合材料在磁场的应用下能有效抑制肿瘤细胞增殖。这一发现表明，磁性静电纺丝壳聚糖纳米纤维复合材料有望用于热疗。
