毒力基因（PsaA 和 CpsA）在肺炎链球菌侵入血液系统中的作用。
Roles of virulence genes (PsaA and CpsA) on the invasion of Streptococcus pneumoniae into blood system.
机构信息
Department of Laboratory Medicine, Taizhou Municipal Hospital, 381 Zhongshan East Road, Jiaojiang District, Taizhou City, Zhejiang Province 318000, China.
出版信息
Eur J Med Res. 2013 May 17;18(1):14. doi: 10.1186/2047-783X-18-14.
BACKGROUND
Streptococcus pneumoniae (SP) is the major cause of childhood mortality worldwide, we need to understand virulence genes of SP so can better target the treatment.We investigated the expression of virulence genes PsaA and CpsA in different strains of SP interacting with monocyte cell line (THP-1) or pneumocyte cell line (A549) and the possible mechanism of SP invasion of the blood system.
METHODS
A total of 23 strains of SP were collected from hospitalized patients (blood-derived and sputum-derived) in the Second Affiliated Hospital of Wenzhou Medical College. The strains and ATCC 49619 were cultured, and RNAs were extracted. THP-1 and A549 cells were stimulated by different SP and ATCC 49619 for 4 h and 8 h, respectively. Quantitative real-time PCR was used to analyze the mRNA expression of PsaA and CpsA. The data were analyzed by SPSS 17.0.
RESULTS
The mRNA level of PsaA and CpsA were all significantly increased in clinical SP strains when compared to ATCC49619 after tedTHP-1 and A549 cells were stimulated. Clinical SPs showed higher virulence compared with ATCC49619.
CONCLUSIONS
The expression of CpsA is the basis of the pathogenicity of SP. The expression of virulence gene PsaA may be helpful to the invasion of SP to the blood system.
背景
肺炎链球菌（SP）是全球儿童死亡的主要原因，我们需要了解 SP 的毒力基因，以便更好地进行针对性治疗。本研究旨在探讨不同 SP 菌株与单核细胞系（THP-1）或肺腺癌细胞系（A549）相互作用时毒力基因 PsaA 和 CpsA 的表达情况及其可能的侵袭血流系统的机制。
方法
从温州医科大学附属第二医院住院患者（血源和痰源）中收集了 23 株 SP 菌株和 ATCC49619。培养菌株并提取 RNA。用不同的 SP 和 ATCC49619 分别刺激 THP-1 和 A549 细胞 4 小时和 8 小时，采用实时定量 PCR 分析 PsaA 和 CpsA 的 mRNA 表达。使用 SPSS 17.0 对数据进行分析。
结果
与 ATCC49619 相比，THP-1 和 A549 细胞刺激后，临床 SP 株中 PsaA 和 CpsA 的 mRNA 水平均显著升高。与 ATCC49619 相比，临床 SP 显示出更高的毒力。
结论
CpsA 的表达是 SP 致病性的基础。毒力基因 PsaA 的表达可能有助于 SP 侵袭血流系统。
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