Burnett R W, Covington A K, Fogh-Andersen N, Külpmann W R, Maas A H, Müller-Plathe O, Siggaard-Andersen O, VanKessel A, Wimberley P D, Zijlstra W G
Department of Pathology, Hartford Hospital, CT, USA.
Eur J Clin Chem Clin Biochem. 1997 Apr;35(4):345-9.
In principle, flame photometry measures substance concentration, and ion-selective electrodes (ISEs) measure ion activity. However, the situation regarding the comparison of results from the two techniques when applied to blood plasma is complex. The problem can be approached experimentally from the point of view of calibration of ion-selective electrodes with concentration calibrators, and similar procedures are adopted for commercial ISE-based clinical analysers. Nevertheless, there is interest in the evaluation of single ion activities in blood plasma and solutions simulating its ionic composition. Solutions are proposed for calibrating ion-selective electrodes for the determination of sodium, potassium and calcium. It is recommended that the values for single ion activities derived from the Pitzer treatment of mixed electrolyte solutions be adopted, because, although this has some empirical features, it has a sounder theoretical basis than the previously used Stokes-Robinson-Bates hydration approach.
原则上，火焰光度法测量物质浓度，而离子选择电极（ISE）测量离子活度。然而，将这两种技术应用于血浆时，比较两者结果的情况很复杂。从用浓度校准物校准离子选择电极的角度，可以通过实验来解决这个问题，基于ISE的商用临床分析仪也采用类似程序。尽管如此，人们仍对评估血浆及模拟其离子组成的溶液中的单个离子活度感兴趣。有人提出了用于校准测定钠、钾和钙的离子选择电极的溶液。建议采用由皮策（Pitzer）混合电解质溶液处理得出的单个离子活度值，因为尽管这有一些经验性特征，但它比以前使用的斯托克斯 - 罗宾逊 - 贝茨（Stokes-Robinson-Bates）水合作用方法有更坚实的理论基础。
