Bou Ghanem Elsa N, Myers-Morales Tanya, Jones Grant S, D'Orazio Sarah E F
Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, USA.
J Vis Exp. 2013 May 6(75):e50381. doi: 10.3791/50381.
L. monocytogenes are facultative intracellular bacterial pathogens that cause food borne infections in humans. Very little is known about the gastrointestinal phase of listeriosis due to the lack of a small animal model that closely mimics human disease. This paper describes a novel mouse model for oral transmission of L. monocytogenes. Using this model, mice fed L. monocytogenes-contaminated bread have a discrete phase of gastrointestinal infection, followed by varying degrees of systemic spread in susceptible (BALB/c/By/J) or resistant (C57BL/6) mouse strains. During the later stages of the infection, dissemination to the gall bladder and brain is observed. The food borne model of listeriosis is highly reproducible, does not require specialized skills, and can be used with a wide variety of bacterial isolates and laboratory mouse strains. As such, it is the ideal model to study both virulence strategies used by L. monocytogenes to promote intestinal colonization, as well as the host response to invasive food borne bacterial infection.
单核细胞增生李斯特菌是兼性胞内细菌病原体，可引起人类食源性感染。由于缺乏能紧密模拟人类疾病的小动物模型，人们对李斯特菌病的胃肠道阶段知之甚少。本文描述了一种用于单核细胞增生李斯特菌经口传播的新型小鼠模型。使用该模型，喂食受单核细胞增生李斯特菌污染面包的小鼠会经历一个离散的胃肠道感染阶段，随后在易感（BALB/c/By/J）或抗性（C57BL/6）小鼠品系中出现不同程度的全身扩散。在感染后期，可观察到细菌扩散至胆囊和脑部。李斯特菌病的食源性模型具有高度可重复性，不需要专门技能，并且可用于多种细菌分离株和实验室小鼠品系。因此，它是研究单核细胞增生李斯特菌促进肠道定植所采用的毒力策略以及宿主对侵袭性食源性细菌感染反应的理想模型。
