Bou Ghanem Elsa N, Myers-Morales Tanya, D'Orazio Sarah E F
Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky.
Curr Protoc Microbiol. 2013 Nov 5;31:9B.3.1-9B.3.16. doi: 10.1002/9780471729259.mc09b03s31.
Listeria monocytogenes causes foodborne disease in humans that ranges in severity from mild, self-limiting gastroenteritis to life-threatening systemic infections of the blood, brain, or placenta. The most commonly used animal model of listeriosis is intravenous infection of mice. This systemic model is highly reproducible, and thus, useful for studying cell-mediated immune responses against an intracellular bacterial pathogen, but it completely bypasses the gastrointestinal phase of L. monocytogenes infection. Intragastric inoculation of L. monocytogenes produces more variable results and may cause direct bloodstream invasion in some animals. The foodborne transmission model described here does not require specialized skills to perform and results in infections that more closely mimic human disease. This natural feeding model can be used to study both the host- and pathogen-derived factors that govern susceptibility or resistance to orally acquired L. monocytogenes.
单核细胞增生李斯特菌可引发人类食源性疾病，其严重程度从轻微的自限性肠胃炎到危及生命的血液、脑部或胎盘的全身性感染不等。最常用的李斯特菌病动物模型是对小鼠进行静脉感染。这种全身性模型具有高度可重复性，因此，对于研究针对细胞内细菌病原体的细胞介导免疫反应很有用，但它完全绕过了单核细胞增生李斯特菌感染的胃肠道阶段。对单核细胞增生李斯特菌进行胃内接种会产生更具变数的结果，并且在某些动物中可能导致直接的血流侵袭。此处描述的食源性传播模型不需要专门技能即可操作，并且所导致的感染更接近模拟人类疾病。这种自然喂养模型可用于研究决定对经口获得的单核细胞增生李斯特菌易感性或抵抗力的宿主和病原体衍生因素。
