Department of Otolaryngology-Head and Neck Surgery, West China Hospital, Sichuan University, 37, Guoxue Lane, Chengdu, 610041, China.
Pathol Oncol Res. 2013 Oct;19(4):771-7. doi: 10.1007/s12253-013-9642-0. Epub 2013 May 19.
Beclin-1 plays a critical role in the regulation of autophagy, apoptosis, differentiation and the development and progression of cancer. The aim of the present investigation was to analyze the Beclin-1 protein expression and to assess its prognostic significance in tissue of laryngeal squamous cell carcinoma (LSCC). Beclin-1 protein expression in 82 primary laryngeal squamous cell carcinoma and 40 paracarcinoma non-tumor tissue samples was analyzed by immunohistochemistry and correlated with clinicopathological parameters and patients' outcome. The expression of Beclin-1 in tumor tissues was significantly lower than that in non-tumor tissues (P = 0.035). Reduced Beclin-1 expression was significantly correlated with lymph node metastases (P = 0.021). Kaplan-Meier survival estimates showed a significant correlation between Beclin-1 expression and patient's survival rate (log-rank P < 0.05). Multivariate Cox proportional hazards model analysis confirmed that lymph node metastases (P = 0.048) and Beclin-1 expression (P = 0.029) were statistically significant, independent prognostic factors for LSCC. Our findings suggested that decreased Beclin-1 expression and lymph node metastases, as examined by immunohistochemistry, are both independent biomarker for poor prognosis of patients with LSCC.
Beclin-1 在自噬、细胞凋亡、分化以及癌症的发生和发展中起着关键作用。本研究旨在分析 Beclin-1 蛋白的表达，并评估其在喉鳞状细胞癌（LSCC）组织中的预后意义。采用免疫组织化学法检测 82 例原发性喉鳞状细胞癌和 40 例癌旁非肿瘤组织样本中的 Beclin-1 蛋白表达，并与临床病理参数及患者预后进行相关性分析。结果显示，肿瘤组织中 Beclin-1 的表达明显低于非肿瘤组织（P = 0.035）。Beclin-1 表达降低与淋巴结转移显著相关（P = 0.021）。Kaplan-Meier 生存估计表明，Beclin-1 表达与患者生存率之间存在显著相关性（对数秩 P < 0.05）。多因素 Cox 比例风险模型分析证实，淋巴结转移（P = 0.048）和 Beclin-1 表达（P = 0.029）是 LSCC 的独立预后因素。这些结果表明，免疫组织化学检测到的 Beclin-1 表达降低和淋巴结转移是 LSCC 患者预后不良的独立生物标志物。
